6qu8: Difference between revisions
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==Adenovirus Serotype 26 (Ad26) in complex with sialic acid, pH8.0== | ==Adenovirus Serotype 26 (Ad26) in complex with sialic acid, pH8.0== | ||
<StructureSection load='6qu8' size='340' side='right'caption='[[6qu8]]' scene=''> | <StructureSection load='6qu8' size='340' side='right'caption='[[6qu8]], [[Resolution|resolution]] 1.19Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QU8 OCA]. For a <b>guided tour on the structure components</b> use [ | <table><tr><td colspan='2'>[[6qu8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_adenovirus_26 Human adenovirus 26]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QU8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6QU8 FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.19Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SIA:O-SIALIC+ACID'>SIA</scene>, <scene name='pdbligand=SLB:5-N-ACETYL-BETA-D-NEURAMINIC+ACID'>SLB</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6qu8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qu8 OCA], [https://pdbe.org/6qu8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6qu8 RCSB], [https://www.ebi.ac.uk/pdbsum/6qu8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6qu8 ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/A4ZKM1_9ADEN A4ZKM1_9ADEN] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Adenoviruses are clinically important agents. They cause respiratory distress, gastroenteritis, and epidemic keratoconjunctivitis. As non-enveloped, double-stranded DNA viruses, they are easily manipulated, making them popular vectors for therapeutic applications, including vaccines. Species D adenovirus type 26 (HAdV-D26) is both a cause of EKC and other diseases and a promising vaccine vector. HAdV-D26-derived vaccines are under investigation as protective platforms against HIV, Zika, and respiratory syncytial virus infections and are in phase 3 clinical trials for Ebola. We recently demonstrated that HAdV-D26 does not use CD46 or Desmoglein-2 as entry receptors, while the putative interaction with coxsackie and adenovirus receptor is low affinity and unlikely to represent the primary cell receptor. Here, we establish sialic acid as a primary entry receptor used by HAdV-D26. We demonstrate that removal of cell surface sialic acid inhibits HAdV-D26 infection, and provide a high-resolution crystal structure of HAdV-D26 fiber-knob in complex with sialic acid. | |||
Human adenovirus type 26 uses sialic acid-bearing glycans as a primary cell entry receptor.,Baker AT, Mundy RM, Davies JA, Rizkallah PJ, Parker AL Sci Adv. 2019 Sep 4;5(9):eaax3567. doi: 10.1126/sciadv.aax3567. eCollection 2019 , Sep. PMID:31517055<ref>PMID:31517055</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 6qu8" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Human adenovirus 26]] | |||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Baker AT]] | [[Category: Baker AT]] | ||
Latest revision as of 15:08, 24 January 2024
Adenovirus Serotype 26 (Ad26) in complex with sialic acid, pH8.0Adenovirus Serotype 26 (Ad26) in complex with sialic acid, pH8.0
Structural highlights
FunctionPublication Abstract from PubMedAdenoviruses are clinically important agents. They cause respiratory distress, gastroenteritis, and epidemic keratoconjunctivitis. As non-enveloped, double-stranded DNA viruses, they are easily manipulated, making them popular vectors for therapeutic applications, including vaccines. Species D adenovirus type 26 (HAdV-D26) is both a cause of EKC and other diseases and a promising vaccine vector. HAdV-D26-derived vaccines are under investigation as protective platforms against HIV, Zika, and respiratory syncytial virus infections and are in phase 3 clinical trials for Ebola. We recently demonstrated that HAdV-D26 does not use CD46 or Desmoglein-2 as entry receptors, while the putative interaction with coxsackie and adenovirus receptor is low affinity and unlikely to represent the primary cell receptor. Here, we establish sialic acid as a primary entry receptor used by HAdV-D26. We demonstrate that removal of cell surface sialic acid inhibits HAdV-D26 infection, and provide a high-resolution crystal structure of HAdV-D26 fiber-knob in complex with sialic acid. Human adenovirus type 26 uses sialic acid-bearing glycans as a primary cell entry receptor.,Baker AT, Mundy RM, Davies JA, Rizkallah PJ, Parker AL Sci Adv. 2019 Sep 4;5(9):eaax3567. doi: 10.1126/sciadv.aax3567. eCollection 2019 , Sep. PMID:31517055[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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