8j5y: Difference between revisions

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-'''Unreleased structure'''
-The entry 8j5y is ON HOLD  until Paper Publication
+==Structural and mechanistic insight into ribosomal ITS2 RNA processing by nuclease-kinase machinery==
+<StructureSection load='8j5y' size='340' side='right'caption='[[8j5y]], [[Resolution|resolution]] 3.07&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[8j5y]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8J5Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8J5Y FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.07&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8j5y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8j5y OCA], [https://pdbe.org/8j5y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8j5y RCSB], [https://www.ebi.ac.uk/pdbsum/8j5y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8j5y ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/A0A8H4BWT6_YEASX A0A8H4BWT6_YEASX]
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Precursor ribosomal RNA (pre-rRNA) processing is a key step in ribosome biosynthesis and involves numerous RNases. A HEPN (higher eukaryote and prokaryote nucleotide binding) nuclease Las1 and a polynucleotide kinase Grc3 assemble into a tetramerase responsible for rRNA maturation. Here, we report the structures of full-length Saccharomyces cerevisiae and Cyberlindnera jadinii Las1-Grc3 complexes, and C. jadinii Las1. The Las1-Grc3 structures show that the central coiled-coil domain of Las1 facilitates pre-rRNA binding and cleavage, while the Grc3 C-terminal loop motif directly binds to the HEPN active center of Las1 and regulates pre-rRNA cleavage. Structural comparison between Las1 and Las1-Grc3 complex exhibits that Grc3 binding induces conformational rearrangements of catalytic residues associated with HEPN nuclease activation. Biochemical assays identify that Las1 processes pre-rRNA at the two specific sites (C2 and C2'), which greatly facilitates rRNA maturation. Our structures and specific pre-rRNA cleavage findings provide crucial insights into the mechanism and pathway of pre-rRNA processing in ribosome biosynthesis.
-Authors: Chen, J., Chen, H., Li, S., Lin, X., Hu, R., Zhang, K., Liu, L.
+Structural and mechanistic insights into ribosomal ITS2 RNA processing by nuclease-kinase machinery.,Chen J, Chen H, Li S, Lin X, Hu R, Zhang K, Liu L Elife. 2024 Jan 5;12:RP86847. doi: 10.7554/eLife.86847. PMID:38180340<ref>PMID:38180340</ref>
-Description: Structural and mechanistic insight into ribosomal ITS2 RNA processing by nuclease-kinase machinery
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Lin, X]]
+<div class="pdbe-citations 8j5y" style="background-color:#fffaf0;"></div>
-[[Category: Li, S]]
+== References ==
-[[Category: Liu, L]]
+<references/>
-[[Category: Chen, J]]
+__TOC__
-[[Category: Hu, R]]
+</StructureSection>
-[[Category: Zhang, K]]
+[[Category: Large Structures]]
-[[Category: Chen, H]]
+[[Category: Saccharomyces cerevisiae]]
+[[Category: Chen H]]
+[[Category: Chen J]]
+[[Category: Hu R]]
+[[Category: Li S]]
+[[Category: Lin X]]
+[[Category: Liu L]]
+[[Category: Zhang K]]