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==Structure of retromer VPS29-VPS35C subunits complexed with RidL N-terminal domain (1-236)== | ==Structure of retromer VPS29-VPS35C subunits complexed with RidL N-terminal domain (1-236)== | ||
<StructureSection load='5osh' size='340' side='right' caption='[[5osh]], [[Resolution|resolution]] 4.30Å' scene=''> | <StructureSection load='5osh' size='340' side='right'caption='[[5osh]], [[Resolution|resolution]] 4.30Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[5osh]] is a 12 chain structure with sequence from [ | <table><tr><td colspan='2'>[[5osh]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Legionella_pneumophila_subsp._pneumophila_ATCC_43290 Legionella pneumophila subsp. pneumophila ATCC 43290]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OSH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5OSH FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 4.3Å</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5osh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5osh OCA], [https://pdbe.org/5osh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5osh RCSB], [https://www.ebi.ac.uk/pdbsum/5osh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5osh ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
[ | [https://www.uniprot.org/uniprot/VPS29_HUMAN VPS29_HUMAN] Essential component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Also required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA). Has low protein phosphatase activity towards a serine-phosphorylated peptide derived from IGF2R (in vitro).<ref>PMID:15247922</ref> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
| Line 22: | Line 20: | ||
==See Also== | ==See Also== | ||
*[[Vacuolar protein sorting-associated protein|Vacuolar protein sorting-associated protein]] | *[[Vacuolar protein sorting-associated protein 3D structures|Vacuolar protein sorting-associated protein 3D structures]] | ||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Homo sapiens]] | ||
[[Category: Legionella pneumophila subsp. pneumophila | [[Category: Large Structures]] | ||
[[Category: Hierro | [[Category: Legionella pneumophila subsp. pneumophila ATCC 43290]] | ||
[[Category: Isupov | [[Category: Hierro A]] | ||
[[Category: Lucas | [[Category: Isupov MN]] | ||
[[Category: Rojas | [[Category: Lucas M]] | ||
[[Category: Romano-Moreno | [[Category: Rojas AL]] | ||
[[Category: Romano-Moreno M]] | |||
Latest revision as of 15:58, 20 December 2023
Structure of retromer VPS29-VPS35C subunits complexed with RidL N-terminal domain (1-236)Structure of retromer VPS29-VPS35C subunits complexed with RidL N-terminal domain (1-236)
Structural highlights
FunctionVPS29_HUMAN Essential component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Also required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA). Has low protein phosphatase activity towards a serine-phosphorylated peptide derived from IGF2R (in vitro).[1] Publication Abstract from PubMedMicrobial pathogens employ sophisticated virulence strategies to cause infections in humans. The intracellular pathogen Legionella pneumophila encodes RidL to hijack the host scaffold protein VPS29, a component of retromer and retriever complexes critical for endosomal cargo recycling. Here, we determined the crystal structure of L. pneumophila RidL in complex with the human VPS29-VPS35 retromer subcomplex. A hairpin loop protruding from RidL inserts into a conserved pocket on VPS29 that is also used by cellular ligands, such as Tre-2/Bub2/Cdc16 domain family member 5 (TBC1D5) and VPS9-ankyrin repeat protein for VPS29 binding. Consistent with the idea of molecular mimicry in protein interactions, RidL outcompeted TBC1D5 for binding to VPS29. Furthermore, the interaction of RidL with retromer did not interfere with retromer dimerization but was essential for association of RidL with retromer-coated vacuolar and tubular endosomes. Our work thus provides structural and mechanistic evidence into how RidL is targeted to endosomal membranes. Molecular mechanism for the subversion of the retromer coat by the Legionella effector RidL.,Romano-Moreno M, Rojas AL, Williamson CD, Gershlick DC, Lucas M, Isupov MN, Bonifacino JS, Machner MP, Hierro A Proc Natl Acad Sci U S A. 2017 Dec 11. pii: 1715361115. doi:, 10.1073/pnas.1715361115. PMID:29229824[2] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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