7k5l: Difference between revisions
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==== | ==Ebola virus VP40 octameric ring generated by an RNA oligonucleotide== | ||
<StructureSection load='7k5l' size='340' side='right'caption='[[7k5l]]' scene=''> | <StructureSection load='7k5l' size='340' side='right'caption='[[7k5l]], [[Resolution|resolution]] 1.38Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br> | <table><tr><td colspan='2'>[[7k5l]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Ebola_virus_-_Mayinga,_Zaire,_1976 Ebola virus - Mayinga, Zaire, 1976] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7K5L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7K5L FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7k5l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7k5l OCA], [https://pdbe.org/7k5l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7k5l RCSB], [https://www.ebi.ac.uk/pdbsum/7k5l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7k5l ProSAT]</span></td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.38Å</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7k5l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7k5l OCA], [https://pdbe.org/7k5l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7k5l RCSB], [https://www.ebi.ac.uk/pdbsum/7k5l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7k5l ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/VP40_EBOZM VP40_EBOZM] Promotes virus assembly and budding by interacting with host proteins of the multivesicular body pathway. May facilitate virus budding by interacting with the nucleocapsid and the plasma membrane. Specific interactions with membrane-associated GP and VP24 during the budding process may also occur. The hexamer form seems to be involved in budding. The octamer form binds RNA, and may play a role in genome replication.<ref>PMID:15892969</ref> <ref>PMID:16719918</ref> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The Ebola virus matrix protein VP40 forms distinct structures linked to distinct functions in the virus life cycle. Dimeric VP40 is a structural protein associated with virus assembly, while octameric, ring-shaped VP40 is associated with transcriptional control. In this study, we show that suitable nucleic acid is sufficient to trigger a dynamic transformation of VP40 dimer into the octameric ring. Deep sequencing reveals a binding preference of the VP40 ring for the 3' untranslated region of cellular mRNA and a guanine- and adenine-rich binding motif. Complementary analyses of the nucleic-acid-induced VP40 ring by native mass spectrometry, electron microscopy, and X-ray crystal structures at 1.8 and 1.4 A resolution reveal the stoichiometry of RNA binding, as well as an interface involving a key guanine nucleotide. The host factor-induced structural transformation of protein structure in response to specific RNA triggers in the Ebola virus life cycle presents unique opportunities for therapeutic inhibition. | |||
Cellular mRNA triggers structural transformation of Ebola virus matrix protein VP40 to its essential regulatory form.,Landeras-Bueno S, Wasserman H, Oliveira G, VanAernum ZL, Busch F, Salie ZL, Wysocki VH, Andersen K, Saphire EO Cell Rep. 2021 Apr 13;35(2):108986. doi: 10.1016/j.celrep.2021.108986. PMID:33852858<ref>PMID:33852858</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 7k5l" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Ebola virus - Mayinga, Zaire, 1976]] | |||
[[Category: Homo sapiens]] | |||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: | [[Category: Landeras-Bueno S]] | ||
[[Category: Salie ZL]] | |||
[[Category: Saphire EO]] | |||
[[Category: Wasserman H]] | |||