3fhj: Difference between revisions
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<StructureSection load='3fhj' size='340' side='right'caption='[[3fhj]], [[Resolution|resolution]] 2.65Å' scene=''> | <StructureSection load='3fhj' size='340' side='right'caption='[[3fhj]], [[Resolution|resolution]] 2.65Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[3fhj]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/ | <table><tr><td colspan='2'>[[3fhj]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FHJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3FHJ FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.65Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=TRP:TRYPTOPHAN'>TRP</scene></td></tr> | |||
<tr id=' | |||
< | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3fhj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fhj OCA], [https://pdbe.org/3fhj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3fhj RCSB], [https://www.ebi.ac.uk/pdbsum/3fhj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3fhj ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3fhj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fhj OCA], [https://pdbe.org/3fhj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3fhj RCSB], [https://www.ebi.ac.uk/pdbsum/3fhj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3fhj ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/SYW_GEOSE SYW_GEOSE] | |||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Geobacillus stearothermophilus]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Carter Jr CW]] | |||
[[Category: Carter | [[Category: Delarue M]] | ||
[[Category: Delarue | [[Category: Kapustina M]] | ||
[[Category: Kapustina | [[Category: Koehl P]] | ||
[[Category: Koehl | [[Category: Laowanapiban P]] | ||
[[Category: Laowanapiban | [[Category: Vonrhein C]] | ||
[[Category: Vonrhein | |||
Latest revision as of 09:46, 6 September 2023
Independent saturation of three TrpRS subsites generates a partially-assembled state similar to those observed in molecular simulationsIndependent saturation of three TrpRS subsites generates a partially-assembled state similar to those observed in molecular simulations
Structural highlights
FunctionEvolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedTwo new crystal structures of Bacillus stearothermophilus tryptophanyl-tRNA synthetase (TrpRS) afford evidence that a closed interdomain hinge angle requires a covalent bond between AMP and an occupant of either pyrophosphate or tryptophan subsite. They also are within experimental error of a cluster of structures observed in a nonequilibrium molecular dynamics simulation showing partial active-site assembly. Further, the highest energy structure in a minimum action pathway computed by using elastic network models for Open and Pretransition state (PreTS) conformations for the fully liganded TrpRS monomer is intermediate between that simulated structure and a partially disassembled structure from a nonequilibrium molecular dynamics trajectory for the unliganded PreTS. These mutual consistencies provide unexpected validation of inferences drawn from molecular simulations. Independent saturation of three TrpRS subsites generates a partially assembled state similar to those observed in molecular simulations.,Laowanapiban P, Kapustina M, Vonrhein C, Delarue M, Koehl P, Carter CW Jr Proc Natl Acad Sci U S A. 2009 Feb 10;106(6):1790-5. Epub 2009 Jan 27. PMID:19174517[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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