2f8t: Difference between revisions
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<StructureSection load='2f8t' size='340' side='right'caption='[[2f8t]], [[Resolution|resolution]] 3.10Å' scene=''> | <StructureSection load='2f8t' size='340' side='right'caption='[[2f8t]], [[Resolution|resolution]] 3.10Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[2f8t]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/ | <table><tr><td colspan='2'>[[2f8t]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F8T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2F8T FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.1Å</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2f8t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f8t OCA], [https://pdbe.org/2f8t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2f8t RCSB], [https://www.ebi.ac.uk/pdbsum/2f8t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2f8t ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2f8t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f8t OCA], [https://pdbe.org/2f8t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2f8t RCSB], [https://www.ebi.ac.uk/pdbsum/2f8t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2f8t ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/O67434_AQUAE O67434_AQUAE] | |||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Aquifex aeolicus | [[Category: Aquifex aeolicus]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Chen | [[Category: Chen HY]] | ||
[[Category: Patel | [[Category: Patel DJ]] | ||
[[Category: Yuan | [[Category: Yuan YR]] | ||
Latest revision as of 12:22, 30 August 2023
Crystal structure of Aa-Ago with externally-bound siRNACrystal structure of Aa-Ago with externally-bound siRNA
Structural highlights
FunctionEvolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedArgonaute proteins are key components of the RNA-induced silencing complex (RISC). They provide both architectural and catalytic functionalities associated with small interfering RNA (siRNA) guide strand recognition and subsequent guide strand-mediated cleavage of complementary mRNAs. We report on the 3.0 A crystal structures of 22-mer and 26-mer siRNAs bound to Aquifex aeolicus Argonaute (Aa-Ago), where one 2 nt 3' overhang of the siRNA inserts into a cavity positioned on the outer surface of the PAZ-containing lobe of the bilobal Aa-Ago architecture. The first overhang nucleotide stacks over a tyrosine ring, while the second overhang nucleotide, together with the intervening sugar-phosphate backbone, inserts into a preformed surface cavity. Photochemical crosslinking studies on Aa-Ago with 5-iodoU-labeled single-stranded siRNA and siRNA duplex provide support for this externally bound siRNA-Aa-Ago complex. The structure and biochemical data together provide insights into a protein-RNA recognition event potentially associated with the RISC-loading pathway. A potential protein-RNA recognition event along the RISC-loading pathway from the structure of A. aeolicus Argonaute with externally bound siRNA.,Yuan YR, Pei Y, Chen HY, Tuschl T, Patel DJ Structure. 2006 Oct;14(10):1557-65. PMID:17027504[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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