4rrg: Difference between revisions
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==Editing domain of threonyl-tRNA synthetase from Methanococcus jannaschii with L-Thr3AA== | ==Editing domain of threonyl-tRNA synthetase from Methanococcus jannaschii with L-Thr3AA== | ||
<StructureSection load='4rrg' size='340' side='right' caption='[[4rrg]], [[Resolution|resolution]] 1.93Å' scene=''> | <StructureSection load='4rrg' size='340' side='right'caption='[[4rrg]], [[Resolution|resolution]] 1.93Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4rrg]] is a 4 chain structure with sequence from [ | <table><tr><td colspan='2'>[[4rrg]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii_DSM_2661 Methanocaldococcus jannaschii DSM 2661]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RRG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4RRG FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=A3T:3-DEOXY-3-(L-THREONYLAMINO)ADENOSINE'>A3T</scene | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A3T:3-DEOXY-3-(L-THREONYLAMINO)ADENOSINE'>A3T</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4rrg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rrg OCA], [https://pdbe.org/4rrg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4rrg RCSB], [https://www.ebi.ac.uk/pdbsum/4rrg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4rrg ProSAT]</span></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/SYT_METJA SYT_METJA] | |||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
| Line 19: | Line 18: | ||
</div> | </div> | ||
<div class="pdbe-citations 4rrg" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 4rrg" style="background-color:#fffaf0;"></div> | ||
==See Also== | |||
*[[Aminoacyl tRNA synthetase 3D structures|Aminoacyl tRNA synthetase 3D structures]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: | [[Category: Methanocaldococcus jannaschii DSM 2661]] | ||
[[Category: Ahmad | [[Category: Ahmad S]] | ||
[[Category: Kamarthapu | [[Category: Kamarthapu V]] | ||
[[Category: Sankaranarayanan | [[Category: Sankaranarayanan R]] | ||
[[Category: Yerabham | [[Category: Yerabham ASK]] | ||
Revision as of 11:25, 8 March 2023
Editing domain of threonyl-tRNA synthetase from Methanococcus jannaschii with L-Thr3AAEditing domain of threonyl-tRNA synthetase from Methanococcus jannaschii with L-Thr3AA
Structural highlights
FunctionPublication Abstract from PubMedProofreading modules of aminoacyl-tRNA synthetases are responsible for enforcing a high fidelity during translation of the genetic code. They use strategically positioned side chains for specifically targeting incorrect aminoacyl-tRNAs. Here, we show that a unique proofreading module possessing a D-aminoacyl-tRNA deacylase fold does not use side chains for imparting specificity or for catalysis, the two hallmark activities of enzymes. We show, using three distinct archaea, that a side-chain-stripped recognition site is fully capable of solving a subtle discrimination problem. While biochemical probing establishes that RNA plays the catalytic role, mechanistic insights from multiple high-resolution snapshots reveal that differential remodelling of the catalytic core at the RNA-peptide interface provides the determinants for correct proofreading activity. The functional crosstalk between RNA and protein elucidated here suggests how primordial enzyme functions could have emerged on RNA-peptide scaffolds before recruitment of specific side chains. Specificity and catalysis hardwired at the RNA-protein interface in a translational proofreading enzyme.,Ahmad S, Muthukumar S, Kuncha SK, Routh SB, Yerabham AS, Hussain T, Kamarthapu V, Kruparani SP, Sankaranarayanan R Nat Commun. 2015 Jun 26;6:7552. doi: 10.1038/ncomms8552. PMID:26113036[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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