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'''Crystal Structure of MtaN, the Bacillus subtilis Multidrug Transporter Activator, N-terminus''' | '''Crystal Structure of MtaN, the Bacillus subtilis Multidrug Transporter Activator, N-terminus''' | ||
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[[Category: Godsey, M H.]] | [[Category: Godsey, M H.]] | ||
[[Category: Neyfakh, A A.]] | [[Category: Neyfakh, A A.]] | ||
[[Category: | [[Category: Antiparallel coiled-coil]] | ||
[[Category: | [[Category: Winged helix-turn-helix]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 21:00:56 2008'' | |||
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Revision as of 21:00, 2 May 2008
Crystal Structure of MtaN, the Bacillus subtilis Multidrug Transporter Activator, N-terminus
OverviewOverview
MtaN (Multidrug Transporter Activation, N terminus) is a constitutive, transcriptionally active 109-residue truncation mutant, which contains only the N-terminal DNA-binding and dimerization domains of MerR family member Mta. The 2.75 A resolution crystal structure of apo-MtaN reveals a winged helix-turn-helix protein with a protruding 8-turn helix (alpha5) that is involved in dimerization by the formation of an antiparallel coiled-coil. The hydrophobic core and helices alpha1 through alpha4 are structurally homologous to MerR family member BmrR bound to DNA, whereas one wing (Wing 1) is shifted. Differences between the orientation of alpha5 with respect to the core and the revolution of the antiparallel coiled-coil lead to significantly altered conformations of MtaN and BmrR dimers. These shifts result in a conformation of MtaN that appears to be incompatible with the transcription activation mechanism of BmrR and suggest that additional DNA-induced structural changes are necessary.
About this StructureAbout this Structure
1JBG is a Single protein structure of sequence from Bacillus subtilis. Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure of MtaN, a global multidrug transporter gene activator., Godsey MH, Baranova NN, Neyfakh AA, Brennan RG, J Biol Chem. 2001 Dec 14;276(50):47178-84. Epub 2001 Oct 1. PMID:11581256 Page seeded by OCA on Fri May 2 21:00:56 2008