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'''Crystal structure of the RNA polymerase holoenzyme from Thermus thermophilus at 2.6A resolution''' | '''Crystal structure of the RNA polymerase holoenzyme from Thermus thermophilus at 2.6A resolution''' | ||
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[[Category: Thermus thermophilus]] | [[Category: Thermus thermophilus]] | ||
[[Category: RSGI, RIKEN Structural Genomics/Proteomics Initiative.]] | [[Category: RSGI, RIKEN Structural Genomics/Proteomics Initiative.]] | ||
[[Category: | [[Category: Riken structural genomics/proteomics initiative]] | ||
[[Category: | [[Category: Rna polymerase holoenzyme]] | ||
[[Category: | [[Category: Rsgi]] | ||
[[Category: | [[Category: Structural genomic]] | ||
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Revision as of 20:29, 2 May 2008
Crystal structure of the RNA polymerase holoenzyme from Thermus thermophilus at 2.6A resolution
OverviewOverview
In bacteria, the binding of a single protein, the initiation factor sigma, to a multi-subunit RNA polymerase core enzyme results in the formation of a holoenzyme, the active form of RNA polymerase essential for transcription initiation. Here we report the crystal structure of a bacterial RNA polymerase holoenzyme from Thermus thermophilus at 2.6 A resolution. In the structure, two amino-terminal domains of the sigma subunit form a V-shaped structure near the opening of the upstream DNA-binding channel of the active site cleft. The carboxy-terminal domain of sigma is near the outlet of the RNA-exit channel, about 57 A from the N-terminal domains. The extended linker domain forms a hairpin protruding into the active site cleft, then stretching through the RNA-exit channel to connect the N- and C-terminal domains. The holoenzyme structure provides insight into the structural organization of transcription intermediate complexes and into the mechanism of transcription initiation.
About this StructureAbout this Structure
1IW7 is a Protein complex structure of sequences from Thermus thermophilus. The following page contains interesting information on the relation of 1IW7 with [Catabolite Activator Protein]. Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure of a bacterial RNA polymerase holoenzyme at 2.6 A resolution., Vassylyev DG, Sekine S, Laptenko O, Lee J, Vassylyeva MN, Borukhov S, Yokoyama S, Nature. 2002 Jun 13;417(6890):712-9. Epub 2002 May 8. PMID:12000971 Page seeded by OCA on Fri May 2 20:29:50 2008