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==Crystal Structure of the Mevalonate Kinase from an Archaeon Methanosarcina mazei== | ==Crystal Structure of the Mevalonate Kinase from an Archaeon Methanosarcina mazei== | ||
<StructureSection load='4hac' size='340' side='right' caption='[[4hac]], [[Resolution|resolution]] 1.92Å' scene=''> | <StructureSection load='4hac' size='340' side='right'caption='[[4hac]], [[Resolution|resolution]] 1.92Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4hac]] is a 2 chain structure with sequence from [ | <table><tr><td colspan='2'>[[4hac]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanosarcina_mazei_Go1 Methanosarcina mazei Go1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HAC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4HAC FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4hac FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hac OCA], [https://pdbe.org/4hac PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4hac RCSB], [https://www.ebi.ac.uk/pdbsum/4hac PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4hac ProSAT]</span></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | |||
</table> | </table> | ||
== Function == | == Function == | ||
[ | [https://www.uniprot.org/uniprot/Q8PW39_METMA Q8PW39_METMA] Catalyzes the phosphorylation of (R)-mevalonate (MVA) to (R)-mevalonate 5-phosphate (MVAP). Functions in the mevalonate (MVA) pathway leading to isopentenyl diphosphate (IPP), a key precursor for the biosynthesis of isoprenoid compounds such as archaeal membrane lipids.[HAMAP-Rule:MF_00217] | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
| Line 21: | Line 18: | ||
</div> | </div> | ||
<div class="pdbe-citations 4hac" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 4hac" style="background-color:#fffaf0;"></div> | ||
==See Also== | |||
*[[Mevalonate kinase|Mevalonate kinase]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: | [[Category: Methanosarcina mazei Go1]] | ||
[[Category: Lee | [[Category: Lee KH]] | ||
[[Category: Zhuang | [[Category: Zhuang N]] | ||
Revision as of 11:07, 3 November 2022
Crystal Structure of the Mevalonate Kinase from an Archaeon Methanosarcina mazeiCrystal Structure of the Mevalonate Kinase from an Archaeon Methanosarcina mazei
Structural highlights
FunctionQ8PW39_METMA Catalyzes the phosphorylation of (R)-mevalonate (MVA) to (R)-mevalonate 5-phosphate (MVAP). Functions in the mevalonate (MVA) pathway leading to isopentenyl diphosphate (IPP), a key precursor for the biosynthesis of isoprenoid compounds such as archaeal membrane lipids.[HAMAP-Rule:MF_00217] Publication Abstract from PubMedMevalonate kinase (MVK), which plays an important role in catalysing the biosynthesis of isoprenoid compounds derived from the mevalonate pathway, transforms mevalonate to 5-phosphomevalonate using ATP as a cofactor. Mevalonate kinase from Methanosarcina mazei (MmMVK) was expressed in Escherichia coli, purified and crystallized for structural analysis. Diffraction-quality crystals of MmMVK were obtained by the vapour-diffusion method using 0.32 M MgCl(2), 0.08 M bis-tris pH 5.5, 16%(w/v) PEG 3350. The crystals belonged to space group P2(1)2(1)2, with unit-cell parameters a = 97.11, b = 135.92, c = 46.03 A. Diffraction data were collected to 2.08 A resolution. Crystallization and preliminary X-ray diffraction analysis of mevalonate kinase from Methanosarcina mazei.,Zhuang N, Seo KH, Chen C, Zhou J, Kim SW, Lee KH Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Dec 1;68(Pt 12):1560-3., doi: 10.1107/S1744309112047070. Epub 2012 Nov 28. PMID:23192048[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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