5w4t: Difference between revisions
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==Crystal Structure of Fish Cadherin-23 EC1-3== | ==Crystal Structure of Fish Cadherin-23 EC1-3== | ||
<StructureSection load='5w4t' size='340' side='right' caption='[[5w4t]], [[Resolution|resolution]] 2.65Å' scene=''> | <StructureSection load='5w4t' size='340' side='right'caption='[[5w4t]], [[Resolution|resolution]] 2.65Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[5w4t]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Brachidanio_rerio Brachidanio rerio]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5W4T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5W4T FirstGlance]. <br> | <table><tr><td colspan='2'>[[5w4t]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Brachidanio_rerio Brachidanio rerio]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5W4T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5W4T FirstGlance]. <br> | ||
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</div> | </div> | ||
<div class="pdbe-citations 5w4t" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 5w4t" style="background-color:#fffaf0;"></div> | ||
==See Also== | |||
*[[Cadherin 3D structures|Cadherin 3D structures]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Brachidanio rerio]] | [[Category: Brachidanio rerio]] | ||
[[Category: Large Structures]] | |||
[[Category: De-la-Torre, P]] | [[Category: De-la-Torre, P]] | ||
[[Category: Sotomayor, M]] | [[Category: Sotomayor, M]] | ||
Revision as of 20:29, 11 December 2019
Crystal Structure of Fish Cadherin-23 EC1-3Crystal Structure of Fish Cadherin-23 EC1-3
Structural highlights
Publication Abstract from PubMedCadherin-23 (CDH23) is an essential component of hair-cell tip links, fine filaments that mediate inner-ear mechanotransduction. The extracellular domain of CDH23 forms about three-fourths of the tip link with 27 extracellular cadherin (EC) repeats that are structurally similar but not identical to each other. Calcium (Ca(2+)) coordination at the EC linker regions is key for tip-link elasticity and function. There are approximately 116 sites in CDH23 affected by deafness-causing mutations, many of which alter conserved Ca(2+)-binding residues. Here we present crystal structures showing 18 CDH23 EC repeats, including the most and least conserved, a fragment carrying disease mutations, and EC repeats with non-canonical Ca(2+)-binding motif sequences and unusual secondary structure. Complementary experiments show deafness mutations' effects on stability and affinity for Ca(2+). Additionally, a model of nine contiguous CDH23 EC repeats reveals helicity and potential parallel dimerization faces. Overall, our studies provide detailed structural insight into CDH23 function in mechanotransduction. Zooming in on Cadherin-23: Structural Diversity and Potential Mechanisms of Inherited Deafness.,Jaiganesh A, De-la-Torre P, Patel AA, Termine DJ, Velez-Cortes F, Chen C, Sotomayor M Structure. 2018 Jun 27. pii: S0969-2126(18)30209-0. doi:, 10.1016/j.str.2018.06.003. PMID:30033219[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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