5tkf: Difference between revisions
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==Neurospora crassa polysaccharide monooxygenase 2 high mannosylation== | ==Neurospora crassa polysaccharide monooxygenase 2 high mannosylation== | ||
<StructureSection load='5tkf' size='340' side='right' caption='[[5tkf]], [[Resolution|resolution]] 2.10Å' scene=''> | <StructureSection load='5tkf' size='340' side='right'caption='[[5tkf]], [[Resolution|resolution]] 2.10Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[5tkf]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Chrysonilia_crassa Chrysonilia crassa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TKF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5TKF FirstGlance]. <br> | <table><tr><td colspan='2'>[[5tkf]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Chrysonilia_crassa Chrysonilia crassa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TKF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5TKF FirstGlance]. <br> | ||
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</div> | </div> | ||
<div class="pdbe-citations 5tkf" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 5tkf" style="background-color:#fffaf0;"></div> | ||
==See Also== | |||
*[[Monooxygenase 3D structures|Monooxygenase 3D structures]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Chrysonilia crassa]] | [[Category: Chrysonilia crassa]] | ||
[[Category: Large Structures]] | |||
[[Category: Dell, W B.O]] | [[Category: Dell, W B.O]] | ||
[[Category: Meilleur, F]] | [[Category: Meilleur, F]] | ||
[[Category: Oxidoreductase]] | [[Category: Oxidoreductase]] | ||
[[Category: Polysaccharide monooxygenase]] | [[Category: Polysaccharide monooxygenase]] | ||
Revision as of 10:53, 27 November 2019
Neurospora crassa polysaccharide monooxygenase 2 high mannosylationNeurospora crassa polysaccharide monooxygenase 2 high mannosylation
Structural highlights
Publication Abstract from PubMedLytic polysaccharide monooxygenases (LPMOs) are carbohydrate-disrupting enzymes secreted by bacteria and fungi that break glycosidic bonds via an oxidative mechanism. Fungal LPMOs typically act on cellulose and can enhance the efficiency of cellulose-hydrolyzing enzymes that release soluble sugars for bioethanol production or other industrial uses. The enzyme PMO-2 from Neurospora crassa (NcPMO-2) was heterologously expressed in Pichia pastoris to facilitate crystallographic studies of the fungal LPMO mechanism. Diffraction resolution and crystal morphology were improved by expressing NcPMO-2 from a glycoengineered strain of P. pastoris and by the use of crystal seeding methods, respectively. These improvements resulted in high-resolution (1.20 A) X-ray diffraction data collection at 100 K and the production of a large NcPMO-2 crystal suitable for room-temperature neutron diffraction data collection to 2.12 A resolution. Crystallization of a fungal lytic polysaccharide monooxygenase expressed from glycoengineered Pichia pastoris for X-ray and neutron diffraction.,O'Dell WB, Swartz PD, Weiss KL, Meilleur F Acta Crystallogr F Struct Biol Commun. 2017 Feb 1;73(Pt 2):70-78. doi:, 10.1107/S2053230X16020318. Epub 2017 Jan 19. PMID:28177316[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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