Phospholipase D: Difference between revisions

Revision as of 16:57, 4 August 2019

Function

Phospholipase D (PLD) hydrolyzes phosphatidylcholine (PC) into phosphatidic acid and choline^[1]. PLD is found in organisms ranging from virus to mammals.

Relevance

Dysregulation of lipid pathways is involved in many neurodegenerative disorders like Alzheimer disease. Brains of Alzheimer disease patients show decreased levels of PC and increased activity of PLD^[2].

Structural highlights

The ^[3]. Water molecule are shown as red sphere.

Phospholipase D complex with substrate dibutyrylphosphatidylcholine (PDB code 1v0y)

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3D structures of phospholipase D3D structures of phospholipase D

Updated on 04-August-2019

4rw3 – bsPLD – brown spider
4rw5 – bsPLD (mutant)
3rlg, 3rlh – PLD (mutant) – Loxosceles intemedia
4q6x – PLD – cave spider
2ze4, 1v0r, 1v0s, 1f0i – SaPLD – Streptomyces antibioticus
1v0v, 1v0w – SaPLD + phosphite
1v0y – SaPLD + phosphatidylcholine derivative
2ze9 – SaPLD (mutant) + phosphatidylcholine
1v0t, 1v0u – SaPLD + glycerophosphate

ReferencesReferences

↑ McDermott M, Wakelam MJ, Morris AJ. Phospholipase D. Biochem Cell Biol. 2004 Feb;82(1):225-53. PMID:15052340 doi:http://dx.doi.org/10.1139/o03-079
↑ Oliveira TG, Di Paolo G. Phospholipase D in brain function and Alzheimer's disease. Biochim Biophys Acta. 2010 Aug;1801(8):799-805. doi:, 10.1016/j.bbalip.2010.04.004. Epub 2010 Apr 23. PMID:20399893 doi:http://dx.doi.org/10.1016/j.bbalip.2010.04.004
↑ Leiros I, McSweeney S, Hough E. The reaction mechanism of phospholipase D from Streptomyces sp. strain PMF. Snapshots along the reaction pathway reveal a pentacoordinate reaction intermediate and an unexpected final product. J Mol Biol. 2004 Jun 11;339(4):805-20. PMID:15165852 doi:http://dx.doi.org/10.1016/j.jmb.2004.04.003

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Michal Harel, Alexander Berchansky, Joel L. Sussman

[1] McDermott M, Wakelam MJ, Morris AJ. Phospholipase D. Biochem Cell Biol. 2004 Feb;82(1):225-53. PMID:15052340 doi:http://dx.doi.org/10.1139/o03-079

[2] Oliveira TG, Di Paolo G. Phospholipase D in brain function and Alzheimer's disease. Biochim Biophys Acta. 2010 Aug;1801(8):799-805. doi:, 10.1016/j.bbalip.2010.04.004. Epub 2010 Apr 23. PMID:20399893 doi:http://dx.doi.org/10.1016/j.bbalip.2010.04.004

[3] Leiros I, McSweeney S, Hough E. The reaction mechanism of phospholipase D from Streptomyces sp. strain PMF. Snapshots along the reaction pathway reveal a pentacoordinate reaction intermediate and an unexpected final product. J Mol Biol. 2004 Jun 11;339(4):805-20. PMID:15165852 doi:http://dx.doi.org/10.1016/j.jmb.2004.04.003

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@@ Line 8: / Line 8: @@
 == Structural highlights ==
-The <scene name='74/749398/Cv/2'>active site of PLD contains the substrate derivative dibutyrylphosphatidylcholine</scene><ref>PMID:15165852</ref>. Water molecule shown as red sphere.
+The <scene name='74/749398/Cv/3'>active site of PLD contains the substrate derivative dibutyrylphosphatidylcholine</scene><ref>PMID:15165852</ref>. Water molecule are shown as red sphere.
 </StructureSection>