4rw3
Structural insights into substrate binding of brown spider venom class II phospholipases DStructural insights into substrate binding of brown spider venom class II phospholipases D
Structural highlights
FunctionA1HB2_LOXIN Catalyzes the hydrolysis of sphingomyelin. May also acts on other phosphatidyl esters. Induces complement-dependent hemolysis, dermonecrosis, blood vessel permeability and platelet aggregation.[1] Publication Abstract from PubMedPhospholipases D (PLDs) the major dermonecrotic factors from brown spider venoms trigger a range of biological reactions both in vitro and in vivo. Despite their clinical relevance in loxoscelism, structural data is restricted to the apo-form of these enzymes, which has been instrumental in understanding the functional differences between the class I and II spider PLDs. The crystal structures of the native class II PLD from Loxosceles intermedia complexed with myo-inositol 1-phosphate and the inactive mutant H12A complexed with fatty acids indicate the existence of a strong ligand-dependent conformation change of the highly conserved aromatic residues, Tyr 223 and Trp225 indicating their roles in substrate binding. These results provided insights into the structural determinants for substrate recognition and binding by class II PLDs. Structural Insights into Substrate Binding of Brown Spider Venom Class II Phospholipases D.,Coronado MA, Ullah A, da Silva LS, Chaves-Moreira D, Vuitika L, Chaim OM, Veiga SS, Chahine J, Murakami MT, Arni RK Curr Protein Pept Sci. 2015 May 5. PMID:25961401[2] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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