4x8y: Difference between revisions
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==Crystal structure of human PGRMC1 cytochrome b5-like domain== | ==Crystal structure of human PGRMC1 cytochrome b5-like domain== | ||
<StructureSection load='4x8y' size='340' side='right' caption='[[4x8y]], [[Resolution|resolution]] 1.95Å' scene=''> | <StructureSection load='4x8y' size='340' side='right'caption='[[4x8y]], [[Resolution|resolution]] 1.95Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4x8y]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4X8Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4X8Y FirstGlance]. <br> | <table><tr><td colspan='2'>[[4x8y]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4X8Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4X8Y FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4x8y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4x8y OCA], [http://pdbe.org/4x8y PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4x8y RCSB], [http://www.ebi.ac.uk/pdbsum/4x8y PDBsum]</span></td></tr> | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PGRMC1, HPR6.6, PGRMC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4x8y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4x8y OCA], [http://pdbe.org/4x8y PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4x8y RCSB], [http://www.ebi.ac.uk/pdbsum/4x8y PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4x8y ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | == Function == | ||
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</div> | </div> | ||
<div class="pdbe-citations 4x8y" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 4x8y" style="background-color:#fffaf0;"></div> | ||
==See Also== | |||
*[[Progesterone receptor|Progesterone receptor]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Human]] | |||
[[Category: Large Structures]] | |||
[[Category: Kabe, Y]] | [[Category: Kabe, Y]] | ||
[[Category: Kobayashi, T]] | [[Category: Kobayashi, T]] | ||
Revision as of 10:14, 29 May 2019
Crystal structure of human PGRMC1 cytochrome b5-like domainCrystal structure of human PGRMC1 cytochrome b5-like domain
Structural highlights
Function[PGRC1_HUMAN] Receptor for progesterone. Publication Abstract from PubMedProgesterone-receptor membrane component 1 (PGRMC1/Sigma-2 receptor) is a haem-containing protein that interacts with epidermal growth factor receptor (EGFR) and cytochromes P450 to regulate cancer proliferation and chemoresistance; its structural basis remains unknown. Here crystallographic analyses of the PGRMC1 cytosolic domain at 1.95 A resolution reveal that it forms a stable dimer through stacking interactions of two protruding haem molecules. The haem iron is five-coordinated by Tyr113, and the open surface of the haem mediates dimerization. Carbon monoxide (CO) interferes with PGRMC1 dimerization by binding to the sixth coordination site of the haem. Haem-mediated PGRMC1 dimerization is required for interactions with EGFR and cytochromes P450, cancer proliferation and chemoresistance against anti-cancer drugs; these events are attenuated by either CO or haem deprivation in cancer cells. This study demonstrates protein dimerization via haem-haem stacking, which has not been seen in eukaryotes, and provides insights into its functional significance in cancer. Haem-dependent dimerization of PGRMC1/Sigma-2 receptor facilitates cancer proliferation and chemoresistance.,Kabe Y, Nakane T, Koike I, Yamamoto T, Sugiura Y, Harada E, Sugase K, Shimamura T, Ohmura M, Muraoka K, Yamamoto A, Uchida T, Iwata S, Yamaguchi Y, Krayukhina E, Noda M, Handa H, Ishimori K, Uchiyama S, Kobayashi T, Suematsu M Nat Commun. 2016 Mar 18;7:11030. doi: 10.1038/ncomms11030. PMID:26988023[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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