Adenosine dimethyltransferase

Function Adenosine dimethyltransferase (KsgA) dimethylates two adjacent adenines at the C-terminal of the 16S rRNA in the small 30S ribosomal subunit, thus modifying it. KsgA catalyzes the dimethylation of adenines 1518-1519 using S-adenosyl-methionine (SAM) as the methyl donor and producing S-adenosyl-homocysteine (SAH). Relevance KsgA confers resistance to the antibiotic kasugomycin. Structural highlights Among methyltransferases, KsgA and the reaction it catalyzes are conserved throughout evolution. However, the specifics of substrate recognition by the enzyme remain unknown. Here we report structures of (SAH, reaction product of cofactor S-adenosylmethionine), revealing critical structural information on KsgA-RNA and KsgA-SAH interactions. Moreover, the structures show how conformational changes that occur upon RNA binding create the cofactor-binding site. There are nine conserved functional motifs (motifs I-VIII and X) in KsgA. Prior to RNA binding, motifs I and VIII are flexible, each exhibiting two distinct conformations. Upon RNA binding, the two motifs become stabilized in one of these conformations, which is compatible with the binding of SAH. Motif X, which is also stabilized upon RNA binding, is directly involved in the binding of SAH. 3D structures of adenosine dimethyltransferase Adenosine dimethyltransferase 3D structures

spinqualitylabelsall models Structure of adenosine dimethyltransferase complex with RNA, SAH and K+ ion (purple) (PDB code 3ftf). Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

3D structures of adenosine dimethyltransferase3D structures of adenosine dimethyltransferase

Updated on 28-February-2019

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• Adenosine dimethyltransferase
  • 1qyr – EcKsgA – Escherichia coli
    
  • 3tpz – EcKsgA (mutant)
    
  • 3ftc, 3ftd – AaKsgA – Aquifex aeolicus
    
  • 3fut, 3fuv – TtKsgA – Thermus thermophilus
    
  • 3tqs – KsgA – Coxiella burnetii
    
  • 3uzu – KsgA – Burkholderia pseudomallei
    
  • 4jxj – KsgA – Rickettsia bellii
    
  • 6ifs, 6ifv, 6ifw, 6ifx – BsKsgA – Bacillus subtilis
    
• Adenosine dimethyltransferase complex
  • 3fte – AaKsgA + RNA
    
  • 3ftf – AaKsgA + RNA + SAH
    
  • 3fuu, 3fuw, 3fux – TtKsgA + adenosine derivative
    
  • 3grr – MjKsgA (mutant) + SAH – Methanocaldococcus jannaschii
    
  • 3gru – MjKsgA (mutant) + AMP
    
  • 3grv – MjKsgA (mutant) + adenosine
    
  • 3gry – MjKsgA (mutant) + SAM
    
  • 3r9x – AaKsgA + GDPNP + RNA + GTPase
    
  • 4adv – EcKsgA + 30S ribosomal subunit – Cryo-EM
    
  • 4lf4, 4lf5, 4lf6, 4lf7, 4lf8, 4lf9, 4lfa, 4lfb, 4lfc – TtKsgA + 30S ribosomal subunit
    
  • 6ift – SbKsgA + SAM