Pseudouridine synthase

Pseudouridine is an abundant modified nucleoside found in RNA molecules. It is formed post-transcriptionally in RNA molecules by isomerization of the bond between base and ribose in uridine. There are two major types of enzymes that catalyze this reaction: RNA-guided pseudouridine synthase -- Some ribonucleoproteins (complexes of proteins and small RNAs) have been identified in archaea and eukaryotes to catalyze these reactions. The small RNA in the complex serves as a guide element to select the site of modification through base-pairing with the target RNA molecule. Guide-independent pseudouridine synthase -- The alternative to using the RNA-guided mechanism for pseudouridine formation is a guide-independent mechanism acting through protein-only enzymes to catalyze the isomerization. Structural highlights The conversion of uridine to pseudouridine is accompanied by a conformational change of pseudouridine synthase. (1ze2).

spinqualitylabelsall models tRNA pseudouridine synthase complex with RNA that contains pseudouridine monophosphate 1ze2 Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

3D Structures of pseudouridine synthase3D Structures of pseudouridine synthase

Updated on 05-February-2019

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• Pseudouridine synthase
  • 1dj0 – EcPUS I – Escherichia coli
    
  • 1ze1 – TmPUS B – Thermotoga maritima
    
  • 1ksk, 1ksl – EcPUS RsuA
    
  • 1vio - PUS RsuA – Haemophilus influenzae
    
  • 1xpi - EcPUS RluC catalytic domain
    
  • 1v9k - EcPUS RluC C-terminal domain (mutant)
    
  • 2ist - EcPUS RluD
    
  • 1prz, 1qyu, 1v9f - EcPUS RluD catalytic domain
    
  • 2olw - EcPUS RluE
    
  • 2oml - EcPUS RluE catalytic domain
    
  • 2gml - EcPUS RluF catalytic domain
    
  • 4iqm, 4its, 4j37, 4nz6 - hPUS Pus1 catalytic domain - human
    
  • 4nz7 - hPUS Pus1 catalytic domain (mutant)
    
  • 1vs3 – PUS TruA – Thermus thermophilus
    
  • 1sgv – PUS TruB – Mycobacterium tuberculosis
    
  • 1si7, 1szw, 1sb7 - EcPUS TruD
    
• Pseudouridine synthase complex with RNA
  • 1k8w – EcPUS B + stem-loop RNA
    
  • 1zl3 - EcPUS B (mutant) + stem-loop RNA
    
  • 2i82 – EcPUS RluA + RNA
    
  • 3dh3 - EcPUS RluF + RNA
    
  • 2nqp, 2nr0, 2nre – EcPUS TruA + Leu-tRNA
    
  • 3hjw, 3lwo, 3lwp, 3lwq, 3lwr, 3lwv – PfPUS Cbf5 + ribosome biogenesis protein Nop10 + ribosomal protein L7 + RNA – Pyrococcus furiosus
    
  • 3mqk - PfPUS Cbf5 + ribosome biogenesis protein Nop10 + small nuclear RNP Gar1-like protein + RNA
    
  • 3hjy - PfPUS Cbf5 + ribosome biogenesis protein NOP10 + RNA
    
  • 1ze2 - TmPUS B + RNA
    
  • 2ab4 - TmPUS B (mutant) + RNA
    
  • 1r3e, 1r3f - EcPUS B + RNA
    
• Probable pseudouridine synthase
  • 2apo – PPUS + ribosome biogenesis protein Nop10 – Methanocaldococcus jannaschii
    
  • 2ey4 - PfPPUS Cbf5 + ribosome biogenesis protein Nop10 + small nucleolar RNP
    
  • 2hvy, 3hax - PfPPUS B + ribosome biogenesis protein Nop10 + ribosomal protein L7 + RNA
    
  • 2rfk - PfPUS B + ribosome biogenesis protein Nop10 + small nucleolar RNP + RNA
    
  • 3hay - PfPPUS B + ribosome biogenesis protein Nop10 + ribosomal protein L7 + small nucleolar RNP + RNA
    
  • 2aus – PPUS B + ribosome biogenesis protein Nop10 – Pyrococcus abyssi
    
  • 1z2z – PPUS D – Methanosarcina mazei