Transposase: Difference between revisions
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== Function == | == Function == | ||
'''Transposases''' bind to the ends of a transposon and catalyze its movement to another part of the genome by a cut-and-paste mechanism. Transposase contains a DDE motif in its active site enabling it to coordinate the binding of divalent metal ions. The metal ions affect transposase reactivity. <br /> | '''Transposases''' bind to the ends of a transposon and catalyze its movement to another part of the genome by a cut-and-paste mechanism<ref>PMID:19478801</ref>. Transposase contains a DDE motif in its active site enabling it to coordinate the binding of divalent metal ions. The metal ions affect transposase reactivity. <br /> | ||
*'''Mu transposase''' (MuA) is essential for integration, replication-transposition and excision of bacteriophage Mu DNA into multiple sites of bacterial genome. Mu transposition occurs within the transposome which is a protein-DNA complex which includes 4 subunits of MuA.<br /> | *'''Mu transposase''' (MuA) is essential for integration, replication-transposition and excision of bacteriophage Mu DNA into multiple sites of bacterial genome. Mu transposition occurs within the transposome which is a protein-DNA complex which includes 4 subunits of MuA<ref>PMID:7628012</ref>.<br /> | ||
*'''Hermes transposase''' is a fly protein.<br /> | *'''Hermes transposase''' is a fly protein<ref>PMID:19450689</ref>.<br /> | ||
* '''Dra2 transposase''' is a ''Deinococcus radiodurans'' protein<br /> | * '''Dra2 transposase''' is a ''Deinococcus radiodurans'' protein<br /> | ||
*'''Tn5 transposase''' active site contains the DDE motif which catalyzes the movement of the transposon<br /> | *'''Tn5 transposase''' active site contains the DDE motif which catalyzes the movement of the transposon<ref>PMID:18680433</ref>.<br /> | ||
*'''Mos1 transposase''' mediates the movement of Mos1 transposon<br /> | *'''Mos1 transposase''' mediates the movement of Mos1 transposon<ref>PMID:10762422</ref><br /> | ||
*'''Tc3 transposase''' mediates the movement of Tc3 transposon in ''C. elegans''<br /> | *'''Tc3 transposase''' mediates the movement of Tc3 transposon in ''C. elegans''<ref>PMID:7838706</ref><br /> | ||
== Disease == | == Disease == | ||
Revision as of 12:41, 21 September 2016
FunctionTransposases bind to the ends of a transposon and catalyze its movement to another part of the genome by a cut-and-paste mechanism[1]. Transposase contains a DDE motif in its active site enabling it to coordinate the binding of divalent metal ions. The metal ions affect transposase reactivity.
DiseaseRelevanceStructural highlights |
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3D structures of transposase3D structures of transposase
Updated on 21-September-2016
ReferencesReferences
- ↑ Ivics Z, Li MA, Mates L, Boeke JD, Nagy A, Bradley A, Izsvak Z. Transposon-mediated genome manipulation in vertebrates. Nat Methods. 2009 Jun;6(6):415-22. doi: 10.1038/nmeth.1332. PMID:19478801 doi:http://dx.doi.org/10.1038/nmeth.1332
- ↑ Rice P, Mizuuchi K. Structure of the bacteriophage Mu transposase core: a common structural motif for DNA transposition and retroviral integration. Cell. 1995 Jul 28;82(2):209-20. PMID:7628012
- ↑ Park JM, Evertts AG, Levin HL. The Hermes transposon of Musca domestica and its use as a mutagen of Schizosaccharomyces pombe. Methods. 2009 Nov;49(3):243-7. doi: 10.1016/j.ymeth.2009.05.004. Epub 2009 May, 18. PMID:19450689 doi:http://dx.doi.org/10.1016/j.ymeth.2009.05.004
- ↑ Reznikoff WS. Transposon Tn5. Annu Rev Genet. 2008;42:269-86. PMID:18680433 doi:http://dx.doi.org/10.1146/annurev.genet.42.110807.091656
- ↑ Wang W, Swevers L, Iatrou K. Mariner (Mos1) transposase and genomic integration of foreign gene sequences in Bombyx mori cells. Insect Mol Biol. 2000 Apr;9(2):145-55. PMID:10762422
- ↑ Colloms SD, van Luenen HG, Plasterk RH. DNA binding activities of the Caenorhabditis elegans Tc3 transposase. Nucleic Acids Res. 1994 Dec 25;22(25):5548-54. PMID:7838706