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{{Large structure}} | |||
==Crystal structure of the oligomer formed by the kinase-ribonuclease domain of Ire1== | ==Crystal structure of the oligomer formed by the kinase-ribonuclease domain of Ire1== | ||
<StructureSection load='3fbv' size='340' side='right' caption='[[3fbv]], [[Resolution|resolution]] 3.20Å' scene=''> | <StructureSection load='3fbv' size='340' side='right' caption='[[3fbv]], [[Resolution|resolution]] 3.20Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[3fbv]] is a 14 chain structure with sequence from [http://en.wikipedia.org/wiki/ | <table><tr><td colspan='2'>[[3fbv]] is a 14 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FBV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3FBV FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=APJ:N~2~-1H-BENZIMIDAZOL-5-YL-N~4~-(3-CYCLOPROPYL-1H-PYRAZOL-5-YL)PYRIMIDINE-2,4-DIAMINE'>APJ</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=APJ:N~2~-1H-BENZIMIDAZOL-5-YL-N~4~-(3-CYCLOPROPYL-1H-PYRAZOL-5-YL)PYRIMIDINE-2,4-DIAMINE'>APJ</scene></td></tr> | ||
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene>, <scene name='pdbligand=TPO:PHOSPHOTHREONINE'>TPO</scene></td></tr> | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene>, <scene name='pdbligand=TPO:PHOSPHOTHREONINE'>TPO</scene></td></tr> | ||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">IRE1, ERN1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id= | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">IRE1, ERN1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3fbv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fbv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3fbv RCSB], [http://www.ebi.ac.uk/pdbsum/3fbv PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3fbv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fbv OCA], [http://pdbe.org/3fbv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3fbv RCSB], [http://www.ebi.ac.uk/pdbsum/3fbv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3fbv ProSAT]</span></td></tr> | ||
</table> | </table> | ||
{{Large structure}} | |||
== Function == | == Function == | ||
[[http://www.uniprot.org/uniprot/IRE1_YEAST IRE1_YEAST]] Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices HAC1 precursor mRNA to produce the mature form which then induces transcription of UPR target genes.<ref>PMID:8663458</ref> <ref>PMID:8670804</ref> <ref>PMID:9323131</ref> | [[http://www.uniprot.org/uniprot/IRE1_YEAST IRE1_YEAST]] Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices HAC1 precursor mRNA to produce the mature form which then induces transcription of UPR target genes.<ref>PMID:8663458</ref> <ref>PMID:8670804</ref> <ref>PMID:9323131</ref> | ||
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | </div> | ||
<div class="pdbe-citations 3fbv" style="background-color:#fffaf0;"></div> | |||
==See Also== | ==See Also== | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Baker's yeast]] | ||
[[Category: Egea, P F]] | [[Category: Egea, P F]] | ||
[[Category: Finer-Moore, J]] | [[Category: Finer-Moore, J]] | ||
Revision as of 19:08, 5 August 2016
Warning: this is a large structure, and loading might take a long time or not happen at all.
Crystal structure of the oligomer formed by the kinase-ribonuclease domain of Ire1Crystal structure of the oligomer formed by the kinase-ribonuclease domain of Ire1
Structural highlights
Warning: this is a large structure, and loading might take a long time or not happen at all. Function[IRE1_YEAST] Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices HAC1 precursor mRNA to produce the mature form which then induces transcription of UPR target genes.[1] [2] [3] Publication Abstract from PubMedAberrant folding of proteins in the endoplasmic reticulum activates the bifunctional transmembrane kinase/endoribonuclease Ire1. Ire1 excises an intron from HAC1 messenger RNA in yeasts and Xbp1 messenger RNA in metozoans encoding homologous transcription factors. This non-conventional mRNA splicing event initiates the unfolded protein response, a transcriptional program that relieves the endoplasmic reticulum stress. Here we show that oligomerization is central to Ire1 function and is an intrinsic attribute of its cytosolic domains. We obtained the 3.2-A crystal structure of the oligomer of the Ire1 cytosolic domains in complex with a kinase inhibitor that acts as a potent activator of the Ire1 RNase. The structure reveals a rod-shaped assembly that has no known precedence among kinases. This assembly positions the kinase domain for trans-autophosphorylation, orders the RNase domain, and creates an interaction surface for binding of the mRNA substrate. Activation of Ire1 through oligomerization expands the mechanistic repertoire of kinase-based signalling receptors. The unfolded protein response signals through high-order assembly of Ire1.,Korennykh AV, Egea PF, Korostelev AA, Finer-Moore J, Zhang C, Shokat KM, Stroud RM, Walter P Nature. 2009 Feb 5;457(7230):687-93. Epub 2008 Dec 14. PMID:19079236[4] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)
OCACategories:
- Baker's yeast
- Egea, P F
- Finer-Moore, J
- Korennykh, A V
- Korostelev, A A
- Shokat, K M
- Stroud, R M
- Walter, P
- Zhang, C
- Aminopyrazole
- Apy29
- Atp-binding
- Complex
- Cytoplasmic
- Endoplasmic reticulum
- Glycoprotein
- Hydrolase
- Inhibitor
- Ire1
- Kinase
- Magnesium
- Membrane
- Metal-binding
- Multifunctional enzyme
- Nucleotide-binding
- Oligomer
- Phosphoprotein
- Ribonuclease
- Rnase
- Serine/threonine-protein kinase
- Transcription
- Transcription regulation
- Transferase
- Transmembrane
- Unfolded protein response