4mzf: Difference between revisions
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==Crystal structure of human Spindlin1 bound to histone H3(K4me3-R8me2a) peptide== | ==Crystal structure of human Spindlin1 bound to histone H3(K4me3-R8me2a) peptide== | ||
<StructureSection load='4mzf' size='340' side='right' caption='[[4mzf]], [[Resolution|resolution]] 2.10Å' scene=''> | <StructureSection load='4mzf' size='340' side='right' caption='[[4mzf]], [[Resolution|resolution]] 2.10Å' scene=''> | ||
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4mzg|4mzg]], [[4mzh|4mzh]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4mzg|4mzg]], [[4mzh|4mzh]]</td></tr> | ||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SPIN1, OCR, SPIN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SPIN1, OCR, SPIN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4mzf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mzf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4mzf RCSB], [http://www.ebi.ac.uk/pdbsum/4mzf PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4mzf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mzf OCA], [http://pdbe.org/4mzf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4mzf RCSB], [http://www.ebi.ac.uk/pdbsum/4mzf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4mzf ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | </div> | ||
<div class="pdbe-citations 4mzf" style="background-color:#fffaf0;"></div> | |||
== References == | == References == | ||
<references/> | <references/> | ||
Revision as of 16:28, 4 August 2016
Crystal structure of human Spindlin1 bound to histone H3(K4me3-R8me2a) peptideCrystal structure of human Spindlin1 bound to histone H3(K4me3-R8me2a) peptide
Structural highlights
Function[SPIN1_HUMAN] May play a role in cell-cycle regulation during the transition from gamete to embryo (By similarity). Publication Abstract from PubMedHistone modification patterns and their combinatorial readout have emerged as a fundamental mechanism for epigenetic regulation. Here we characterized Spindlin1 as a histone effector that senses a cis-tail histone H3 methylation pattern involving trimethyllysine 4 (H3K4me3) and asymmetric dimethylarginine 8 (H3R8me2a) marks. Spindlin1 consists of triple tudor-like Spin/Ssty repeats. Cocrystal structure determination established concurrent recognition of H3K4me3 and H3R8me2a by Spin/Ssty repeats 2 and 1, respectively. Both H3K4me3 and H3R8me2a are recognized using an "insertion cavity" recognition mode, contributing to a methylation state-specific layer of regulation. In vivo functional studies suggest that Spindlin1 activates Wnt/beta-catenin signaling downstream from protein arginine methyltransferase 2 (PRMT2) and the MLL complex, which together are capable of generating a specific H3 "K4me3-R8me2a" pattern. Mutagenesis of Spindlin1 reader pockets impairs activation of Wnt target genes. Taken together, our work connects a histone "lysine-arginine" methylation pattern readout by Spindlin1-to-Wnt signaling at the transcriptional level. Molecular basis underlying histone H3 lysine-arginine methylation pattern readout by Spin/Ssty repeats of Spindlin1.,Su X, Zhu G, Ding X, Lee SY, Dou Y, Zhu B, Wu W, Li H Genes Dev. 2014 Mar 15;28(6):622-36. doi: 10.1101/gad.233239.113. Epub 2014 Mar, 3. PMID:24589551[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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