1kcx: Difference between revisions
No edit summary |
No edit summary |
||
| Line 8: | Line 8: | ||
|GENE= crmp1 (8-525) ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus]) | |GENE= crmp1 (8-525) ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus]) | ||
|DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=cd01314 D-HYD], [http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=PRK08323 PRK08323]</span> | |DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=cd01314 D-HYD], [http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=PRK08323 PRK08323]</span> | ||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1kcx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kcx OCA], [http://www.ebi.ac.uk/pdbsum/1kcx PDBsum | |RELATEDENTRY= | ||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1kcx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kcx OCA], [http://www.ebi.ac.uk/pdbsum/1kcx PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1kcx RCSB]</span> | |||
}} | }} | ||
| Line 36: | Line 37: | ||
[[Category: structural genomic]] | [[Category: structural genomic]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:46:45 2008'' | ||
Revision as of 21:46, 30 March 2008
| |||||||
| , resolution 2.12Å | |||||||
|---|---|---|---|---|---|---|---|
| Gene: | crmp1 (8-525) (Mus musculus) | ||||||
| Domains: | D-HYD, PRK08323 | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
X-ray structure of NYSGRC target T-45
OverviewOverview
Collapsin response mediator proteins (CRMPs) are cytosolic phosphoproteins involved in neuronal differentiation and axonal guidance. CRMP2 was previously shown to mediate the repulsive effect of Sema3A on axons and to participate in axonal specification. The X-ray crystal structure of murine CRMP1 was determined at 2.1 A resolution and demonstrates that CRMP1 is a bilobed 'lung-shaped' protein forming a tetrameric assembly. Structure-based mutagenesis of surface-exposed residues was employed to map functional domains. As a rapid assay for CRMP, we exploited a reconstituted Sema3A signaling system in COS-7 cells expressing the receptor components Neuropilin1 and PlexinA1 (NP1/PlexA1). In these cells, CRMP and PlexA1 form a physical complex that is reduced in amount by NP1 but enhanced by Sema3A/NP1. Furthermore, CRMP accelerates Sema3A-induced cell contraction. Alanine substitutions in one domain of CRMP1 produce a constitutively active protein that causes Sema3A-independent COS-7 contraction. This mutant CRMP mimics the DRG neurite outgrowth-inhibiting effects of Sema3A and reduces Sema3A-induced axonal repulsion. These data provide a structural view of CRMP function in Plex-dependent Sema3A signaling.
About this StructureAbout this Structure
1KCX is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.
ReferenceReference
Structural bases for CRMP function in plexin-dependent semaphorin3A signaling., Deo RC, Schmidt EF, Elhabazi A, Togashi H, Burley SK, Strittmatter SM, EMBO J. 2004 Jan 14;23(1):9-22. Epub 2003 Dec 18. PMID:14685275
Page seeded by OCA on Sun Mar 30 21:46:45 2008