Phage integrase: Difference between revisions
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{{STRUCTURE_1z1g| PDB=1z1g | SIZE=400| SCENE=Phage_integrase/Cv/1 |right|CAPTION= Se-Met Enterobacteria phage λ integrase:DNA tetramer complex (Holiday Junction) [[1z1g]] }} | {{STRUCTURE_1z1g| PDB=1z1g | SIZE=400| SCENE=Phage_integrase/Cv/1 |right|CAPTION= Se-Met Enterobacteria phage λ integrase:DNA tetramer complex (Holiday Junction) [[1z1g]] }} | ||
'''Phage integrase''' (PIN) | '''Phage integrase''' (PIN) mediates recombination between DNA recognition sequences - the phage attachment site attP and the bacterial attachment site attB<ref>PMID:14687564</ref>. The '''Tyrosine family PIN''' uses a catalytic tyrosine to mediate strand cleavage while the '''Serine family PIN''' uses a serine residue. The High-Pathogenicity Island (HPI) is a genomic island essential for virulence of the family of Enterobacteria. | ||
== 3D Structures of phage integrase == | == 3D Structures of phage integrase == | ||
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[[1aih]] - PIN catalytic core domain – Haemophilus phage HP1<br /> | [[1aih]] - PIN catalytic core domain – Haemophilus phage HP1<br /> | ||
[[2khv]], [[2kj5]] – PIN residues 102-199 – ''Nitrosospira multiformis'' - NMR | [[2khv]], [[2kj5]] – PIN residues 102-199 – ''Nitrosospira multiformis'' - NMR | ||
== References == | |||
<references/> | |||
[[Category:Topic Page]] | [[Category:Topic Page]] | ||
Revision as of 12:28, 22 June 2016
Phage integrase (PIN) mediates recombination between DNA recognition sequences - the phage attachment site attP and the bacterial attachment site attB[1]. The Tyrosine family PIN uses a catalytic tyrosine to mediate strand cleavage while the Serine family PIN uses a serine residue. The High-Pathogenicity Island (HPI) is a genomic island essential for virulence of the family of Enterobacteria.
3D Structures of phage integrase3D Structures of phage integrase
Update June 2011
3nrw – PIN/site-specific recombinase N-terminal – Haloarcula marismortui
3jtz – PIN arm-type binding domain – Yersinia pestis
3ju0 – HPI PIN arm-type binding domain – Pectobacterium atrosepticum
2kkp – PIN SAM-like domain – Moorella thermoacetica – NMR
2kkv – PIN fragment – Salmonella enterica – NMR
2khq - PIN fragment – Staphylococcus saprophyticus – NMR
2wcc – λPIN DBD+ DNA – Enterobacteria phage λ - NMR
1kjk – λPIN N-terminal - NMR
2oxo, 1z19, 1z1b - λPIN core binding domain + DNA
1ae9 - λPIN catalytic core domain (mutant)
1z1g – λPIN + DNA (Holliday junction)
1p7d – λPIN fragment + DNA
3bvp – PIN catalytic core domain – Lactococcus phage TP101-1
1aih - PIN catalytic core domain – Haemophilus phage HP1
2khv, 2kj5 – PIN residues 102-199 – Nitrosospira multiformis - NMR
ReferencesReferences
- ↑ Groth AC, Calos MP. Phage integrases: biology and applications. J Mol Biol. 2004 Jan 16;335(3):667-78. PMID:14687564 doi:http://dx.doi.org/10.1016/S0022283603013561