Maltose-binding protein: Difference between revisions
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{{STRUCTURE_1n3w| PDB=1n3w | SIZE=400| SCENE= |right|CAPTION=Maltose-binding protein complex with maltose, [[1n3w]] }} | {{STRUCTURE_1n3w| PDB=1n3w | SIZE=400| SCENE= |right|CAPTION=Maltose-binding protein complex with maltose, [[1n3w]] }} | ||
== Function == | |||
'''Maltose-binding protein''' (MBP) or '''ABC maltose transporter''' is part of the maltose/maltodextrin system in ''E. coli'' which is responsible of the catabolism of maltodextrin. MBP binds maltodextrin when the latter diffuses into the periplasm and passes it to proteins involved in active transport<ref>PMID:10452611</ref>. | |||
== Relevance == | |||
MBP is widely used in molecular biology as it adds to the solubility of the proteins expressed in ''E. coli'' by being fused to them. The MBP-fusion protein can be purified by binding it to an amylose column followed by elution with maltose<ref>PMID:21125392</ref>. | |||
== Structural highlights == | |||
==3D structures of maltose-binding protein== | ==3D structures of maltose-binding protein== | ||
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**[[4nuf]] - MBP /nuclear receptor + orphan nuclear receptor peptide<br /> | **[[4nuf]] - MBP /nuclear receptor + orphan nuclear receptor peptide<br /> | ||
}} | }} | ||
== References == | |||
<references/> | |||
[[Category:Topic Page]] | [[Category:Topic Page]] | ||
Revision as of 14:00, 14 April 2016
FunctionFunction
Maltose-binding protein (MBP) or ABC maltose transporter is part of the maltose/maltodextrin system in E. coli which is responsible of the catabolism of maltodextrin. MBP binds maltodextrin when the latter diffuses into the periplasm and passes it to proteins involved in active transport[1].
RelevanceRelevance
MBP is widely used in molecular biology as it adds to the solubility of the proteins expressed in E. coli by being fused to them. The MBP-fusion protein can be purified by binding it to an amylose column followed by elution with maltose[2].
Structural highlightsStructural highlights
3D structures of maltose-binding protein3D structures of maltose-binding protein
Updated on 14-April-2016
ReferencesReferences
- ↑ Kapust RB, Waugh DS. Escherichia coli maltose-binding protein is uncommonly effective at promoting the solubility of polypeptides to which it is fused. Protein Sci. 1999 Aug;8(8):1668-74. PMID:10452611 doi:http://dx.doi.org/10.1110/ps.8.8.1668
- ↑ Sun P, Tropea JE, Waugh DS. Enhancing the solubility of recombinant proteins in Escherichia coli by using hexahistidine-tagged maltose-binding protein as a fusion partner. Methods Mol Biol. 2011;705:259-74. doi: 10.1007/978-1-61737-967-3_16. PMID:21125392 doi:http://dx.doi.org/10.1007/978-1-61737-967-3_16