1gpc: Difference between revisions
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|PDB= 1gpc |SIZE=350|CAPTION= <scene name='initialview01'>1gpc</scene>, resolution 2.200Å | |PDB= 1gpc |SIZE=350|CAPTION= <scene name='initialview01'>1gpc</scene>, resolution 2.200Å | ||
|SITE= | |SITE= | ||
|LIGAND= <scene name='pdbligand=ZN:ZINC ION'>ZN</scene> | |LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> | ||
|ACTIVITY= | |ACTIVITY= | ||
|GENE= GENE 32 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id= | |GENE= GENE 32 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10665 Enterobacteria phage T4]) | ||
|DOMAIN= | |||
|RELATEDENTRY= | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gpc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gpc OCA], [http://www.ebi.ac.uk/pdbsum/1gpc PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1gpc RCSB]</span> | |||
}} | }} | ||
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==About this Structure== | ==About this Structure== | ||
1GPC is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/ | 1GPC is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_t4 Enterobacteria phage t4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GPC OCA]. | ||
==Reference== | ==Reference== | ||
Crystal structure of a replication fork single-stranded DNA binding protein (T4 gp32) complexed to DNA., Shamoo Y, Friedman AM, Parsons MR, Konigsberg WH, Steitz TA, Nature. 1995 Jul 27;376(6538):362-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7630406 7630406] | Crystal structure of a replication fork single-stranded DNA binding protein (T4 gp32) complexed to DNA., Shamoo Y, Friedman AM, Parsons MR, Konigsberg WH, Steitz TA, Nature. 1995 Jul 27;376(6538):362-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7630406 7630406] | ||
[[Category: | [[Category: Enterobacteria phage t4]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Friedman, A M.]] | [[Category: Friedman, A M.]] | ||
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[[Category: Shamoo, Y.]] | [[Category: Shamoo, Y.]] | ||
[[Category: Steitz, T A.]] | [[Category: Steitz, T A.]] | ||
[[Category: single-stranded dna-binding]] | [[Category: single-stranded dna-binding]] | ||
[[Category: ssb]] | [[Category: ssb]] | ||
[[Category: zinc binding domain]] | [[Category: zinc binding domain]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:48:42 2008'' | ||
Revision as of 20:48, 30 March 2008
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| , resolution 2.200Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | GENE 32 (Enterobacteria phage T4) | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CORE GP32, DNA-BINDING PROTEIN
OverviewOverview
The single-stranded DNA (ssDNA) binding protein gp32 from bacteriophage T4 is essential for T4 DNA replication, recombination and repair. In vivo gp32 binds ssDNA as the replication fork advances and stimulates replisome processivity and accuracy by a factor of several hundred. Gp32 binding affects nearly every major aspect of DNA metabolism. Among its important functions are: (1) configuring ssDNA templates for efficient use by the replisome including DNA polymerase; (2) melting out adventitious secondary structures; (3) protecting exposed ssDNA from nucleases; and (4) facilitating homologous recombination by binding ssDNA during strand displacement. We have determined the crystal structure of the gp32 DNA binding domain complexed to ssDNA at 2.2 A resolution. The ssDNA binding cleft comprises regions from three structural subdomains and includes a positively charged surface that runs parallel to a series of hydrophobic pockets formed by clusters of aromatic side chains. Although only weak electron density is seen for the ssDNA, it indicates that the phosphate backbone contacts an electropositive cleft of the protein, placing the bases in contact with the hydrophobic pockets. The DNA mobility implied by the weak electron density may reflect the role of gp32 as a sequence-independent ssDNA chaperone allowing the largely unstructured ssDNA to slide freely through the cleft.
About this StructureAbout this Structure
1GPC is a Single protein structure of sequence from Enterobacteria phage t4. Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure of a replication fork single-stranded DNA binding protein (T4 gp32) complexed to DNA., Shamoo Y, Friedman AM, Parsons MR, Konigsberg WH, Steitz TA, Nature. 1995 Jul 27;376(6538):362-6. PMID:7630406
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