1gpc

CORE GP32, DNA-BINDING PROTEINCORE GP32, DNA-BINDING PROTEIN

Structural highlights

1gpc is a 1 chain structure with sequence from Escherichia virus T4. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.2Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

SSB_BPT4 Single-stranded DNA-binding protein that participates in viral DNA replication, recombination, and repair (Probable). Coats the lagging-strand ssDNA as the replication fork advances (PubMed:9079662). Stimulates the activities of viral DNA polymerase and DnaB-like SF4 replicative helicase, probably via its interaction with the helicase assembly factor (PubMed:14871889). Together with DnaB-like SF4 replicative helicase and the helicase assembly factor, promotes pairing of two homologous DNA molecules containing complementary single-stranded regions and mediates homologous DNA strand exchange (PubMed:11459967). Promotes also the formation of joint molecules (PubMed:9079662). mRNA specific autogenous translational repressor (PubMed:15507125).[HAMAP-Rule:MF_04152]^[1] ^[2] ^[3] ^[4] ^[5]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

References

↑ Bleuit JS, Xu H, Ma Y, Wang T, Liu J, Morrical SW. Mediator proteins orchestrate enzyme-ssDNA assembly during T4 recombination-dependent DNA replication and repair. Proc Natl Acad Sci U S A. 2001 Jul 17;98(15):8298-305. PMID:11459967 doi:10.1073/pnas.131007498
↑ Ma Y, Wang T, Villemain JL, Giedroc DP, Morrical SW. Dual functions of single-stranded DNA-binding protein in helicase loading at the bacteriophage T4 DNA replication fork. J Biol Chem. 2004 Apr 30;279(18):19035-45. PMID:14871889 doi:10.1074/jbc.M311738200
↑ Borjac-Natour JM, Petrov VM, Karam JD. Divergence of the mRNA targets for the Ssb proteins of bacteriophages T4 and RB69. Virol J. 2004 Sep 17;1:4. PMID:15507125 doi:10.1186/1743-422X-1-4
↑ Kong D, Nossal NG, Richardson CC. Role of the bacteriophage T7 and T4 single-stranded DNA-binding proteins in the formation of joint molecules and DNA helicase-catalyzed polar branch migration. J Biol Chem. 1997 Mar 28;272(13):8380-7. PMID:9079662 doi:10.1074/jbc.272.13.8380
↑ Kong D, Nossal NG, Richardson CC. Role of the bacteriophage T7 and T4 single-stranded DNA-binding proteins in the formation of joint molecules and DNA helicase-catalyzed polar branch migration. J Biol Chem. 1997 Mar 28;272(13):8380-7. PMID:9079662 doi:10.1074/jbc.272.13.8380

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OCA

[1] Bleuit JS, Xu H, Ma Y, Wang T, Liu J, Morrical SW. Mediator proteins orchestrate enzyme-ssDNA assembly during T4 recombination-dependent DNA replication and repair. Proc Natl Acad Sci U S A. 2001 Jul 17;98(15):8298-305. PMID:11459967 doi:10.1073/pnas.131007498

[2] Ma Y, Wang T, Villemain JL, Giedroc DP, Morrical SW. Dual functions of single-stranded DNA-binding protein in helicase loading at the bacteriophage T4 DNA replication fork. J Biol Chem. 2004 Apr 30;279(18):19035-45. PMID:14871889 doi:10.1074/jbc.M311738200

[3] Borjac-Natour JM, Petrov VM, Karam JD. Divergence of the mRNA targets for the Ssb proteins of bacteriophages T4 and RB69. Virol J. 2004 Sep 17;1:4. PMID:15507125 doi:10.1186/1743-422X-1-4

[4] Kong D, Nossal NG, Richardson CC. Role of the bacteriophage T7 and T4 single-stranded DNA-binding proteins in the formation of joint molecules and DNA helicase-catalyzed polar branch migration. J Biol Chem. 1997 Mar 28;272(13):8380-7. PMID:9079662 doi:10.1074/jbc.272.13.8380

[5] Kong D, Nossal NG, Richardson CC. Role of the bacteriophage T7 and T4 single-stranded DNA-binding proteins in the formation of joint molecules and DNA helicase-catalyzed polar branch migration. J Biol Chem. 1997 Mar 28;272(13):8380-7. PMID:9079662 doi:10.1074/jbc.272.13.8380

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