Adenosine dimethyltransferase

Function Adenosine dimethyltransferase (KsgA) dimethylates two adjacent adenines at the C-terminal of the 16S rRNA in the small 30S ribosomal subunit, thus modifying it. KsgA catalyzes the dimethylation of adenines 1518-1519 using S-adenosyl-methionine (SAM) as the methyl donor and producing S-adenosyl-homocysteine (SAH). Relevance KsgA confers resistance to the antibiotic kasugomycin. Structural highlights The active site pocket of KsgA contains residues N113 and L114 which are part of motif IV SAM-dependent methyltrasferase sequence. The active site can acomodate both adenosine and methyladenosine.

spinqualitylabelsall models Structure of adenosine dimethyltransferase complex with RNA, K+ ion (purple) and SAH (PDB code 3ftf). Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

3D structures of adenosine dimethyltransferase3D structures of adenosine dimethyltransferase

Updated on 28-October-2015

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• Adenosine dimethyltransferase
  • 1qyr – EcKsgA – Escherichia coli
    
  • 3tpz – EcKsgA (mutant)
    
  • 3ftc, 3ftd – AaKsgA – Aquifex aeolicus
    
  • 3fut, 3fuv – TtKsgA – Thermus thermophilus
    
  • 3tqs – KsgA – Coxiella burnetii
    
  • 3uzu – KsgA – Burkholderia pseudomallei
    
  • 4jxj – KsgA – Rickettsia bellii
    
• Adenosine dimethyltransferase complex
  • 3fte – AaKsgA + RNA
    
  • 3ftf – AaKsgA + RNA + SAH
    
  • 3fuu, 3fuw, 3fux – TtKsgA + adenosine derivative
    
  • 3grr – MjKsgA (mutant) + SAH – Methanocaldococcus jannaschii
    
  • 3gru – MjKsgA (mutant) + AMP
    
  • 3grv – MjKsgA (mutant) + adenosine
    
  • 3gry – MjKsgA (mutant) + SAM
    
  • 3r9x – AaKsgA + GDPNP + RNA + GTPase
    
  • 4adv – EcKsgA + 30S ribosomal subunit – Cryo-EM