1eb0: Difference between revisions
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<StructureSection load='1eb0' size='340' side='right' caption='[[1eb0]], [[Resolution|resolution]] 1.85Å' scene=''> | <StructureSection load='1eb0' size='340' side='right' caption='[[1eb0]], [[Resolution|resolution]] 1.85Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[1eb0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/ | <table><tr><td colspan='2'>[[1eb0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"urobacillus_pasteurii"_miquel_1889 "urobacillus pasteurii" miquel 1889]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EB0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1EB0 FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1ear|1ear]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1ear|1ear]]</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1eb0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eb0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1eb0 RCSB], [http://www.ebi.ac.uk/pdbsum/1eb0 PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1eb0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eb0 OCA], [http://pdbe.org/1eb0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1eb0 RCSB], [http://www.ebi.ac.uk/pdbsum/1eb0 PDBsum]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | </div> | ||
<div class="pdbe-citations 1eb0" style="background-color:#fffaf0;"></div> | |||
==See Also== | ==See Also== | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Urobacillus pasteurii miquel 1889]] | ||
[[Category: Beeumen, J Van]] | [[Category: Beeumen, J Van]] | ||
[[Category: Ciurli, S]] | [[Category: Ciurli, S]] | ||
Revision as of 10:56, 10 September 2015
CRYSTAL STRUCTURE OF BACILLUS PASTEURII UREE AT 1.85 A, PHASED BY SIRAS. TYPE I CRYSTAL FORM.CRYSTAL STRUCTURE OF BACILLUS PASTEURII UREE AT 1.85 A, PHASED BY SIRAS. TYPE I CRYSTAL FORM.
Structural highlights
Function[UREE_BACPA] Involved in urease metallocenter assembly. Binds nickel. Probably functions as a nickel donor during metallocenter assembly.[HAMAP-Rule:MF_00822] Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedBacillus pasteurii UreE (BpUreE) is a putative chaperone assisting the insertion of Ni(2+) ions in the active site of urease. The x-ray structure of the protein has been determined for two crystal forms, at 1.7 and 1.85 A resolution, using SIRAS phases derived from a Hg(2+)-derivative. BpUreE is composed of distinct N- and C-terminal domains, connected by a short flexible linker. The structure reveals the topology of an elongated homodimer, formed by interaction of the two C-terminal domains through hydrophobic interactions. A single Zn(2+) ion bound to four conserved His-100 residues, one from each monomer, connects two dimers resulting in a tetrameric BpUreE known to be formed in concentrated solutions. The Zn(2+) ion can be replaced by Ni(2+) as shown by anomalous difference maps obtained on a crystal of BpUreE soaked in a solution containing NiCl(2). A large hydrophobic patch surrounding the metal ion site is surface-exposed in the biologically relevant dimer. The BpUreE structure represents the first for this class of proteins and suggests a possible role for UreE in the urease nickel-center assembly. Structural basis for Ni(2+) transport and assembly of the urease active site by the metallochaperone UreE from Bacillus pasteurii.,Remaut H, Safarov N, Ciurli S, Van Beeumen J J Biol Chem. 2001 Dec 28;276(52):49365-70. Epub 2001 Oct 15. PMID:11602602[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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