Function
Urease accessory protein (Ure) is a Ni-containing urea hydrolase involved in nitrogen recycling from arginine, ureide and purines. Ure proteins are involved in urease activation by the transfer of Ni into the protein[1].
- UreD - required for nickel incorporation [2].
- UreE - see Molecular Playground/UreE.
- UreF - modulates the activation process of urease by eliminating the binding of Ni irons to noncarbamylated protein[3].
- UreG - modulates the activation process of urease in plants [4].
- UreH - forms in combination with UreF and UreG a pre-activation complex which activates urease[5].
- UreI - probably facilitates nickel incorporation.
Structural highlights
The [6]. Water molecule is shown as red sphere.
3D structures of urease accessory protein
Urease accessory protein 3D structures
References
- ↑ Witte CP, Isidore E, Tiller SA, Davies HV, Taylor MA. Functional characterisation of urease accessory protein G (ureG) from potato. Plant Mol Biol. 2001 Jan;45(2):169-79. PMID:11289508
- ↑ Bacanamwo M, Witte CP, Lubbers MW, Polacco JC. Activation of the urease of Schizosaccharomyces pombe by the UreF accessory protein from soybean. Mol Genet Genomics. 2002 Dec;268(4):525-34. PMID:12471450 doi:10.1007/s00438-002-0769-z
- ↑ Moncrief MB, Hausinger RP. Purification and activation properties of UreD-UreF-urease apoprotein complexes. J Bacteriol. 1996 Sep;178(18):5417-21. PMID:8808930
- ↑ Myrach T, Zhu A, Witte CP. The assembly of the plant urease activation complex and the essential role of the urease accessory protein G (UreG) in delivery of nickel to urease. J Biol Chem. 2017 Sep 1;292(35):14556-14565. doi: 10.1074/jbc.M117.780403. Epub, 2017 Jul 14. PMID:28710280 doi:http://dx.doi.org/10.1074/jbc.M117.780403
- ↑ Fong YH, Wong HC, Chuck CP, Chen YW, Sun H, Wong KB. Assembly of Preactivation Complex for Urease Maturation in Helicobacter pylori: CRYSTAL STRUCTURE OF UreF-UreH PROTEIN COMPLEX. J Biol Chem. 2011 Dec 16;286(50):43241-9. Epub 2011 Oct 19. PMID:22013070 doi:10.1074/jbc.M111.296830
- ↑ Banaszak K, Martin-Diaconescu V, Bellucci M, Zambelli B, Rypniewski W, Maroney MJ, Ciurli S. Crystallographic and X-ray absorption spectroscopic characterization of Helicobacter pylori UreE bound to Ni2+ and Zn2+ reveal a role for the disordered C-terminal arm in metal trafficking. Biochem J. 2011 Oct 20. PMID:22010876 doi:10.1042/BJ20111659