Maltose-binding protein: Difference between revisions
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{{STRUCTURE_1n3w| PDB=1n3w | SIZE=400| SCENE= |right|CAPTION=Maltose-binding protein complex with maltose, [[1n3w]] }} | {{STRUCTURE_1n3w| PDB=1n3w | SIZE=400| SCENE= |right|CAPTION=Maltose-binding protein complex with maltose, [[1n3w]] }} | ||
'''Maltose-binding protein''' (MBP) is part of the maltose/maltodextrin system in ''E. coli'' which is responsible of the catabolism of maltodextrin. MBP binds maltodextrin when the latter diffuses into the periplasm and passes it to proteins involved in active transport. MBP is widely used in molecular biology as it adds to the solubility of the proteins expressed in ''E. coli'' by being fused to them. The MBP-fusion protein can be purified by binding it to an amylose column followed by elution with maltose. | '''Maltose-binding protein''' (MBP) or '''ABC maltose transporter''' is part of the maltose/maltodextrin system in ''E. coli'' which is responsible of the catabolism of maltodextrin. MBP binds maltodextrin when the latter diffuses into the periplasm and passes it to proteins involved in active transport. MBP is widely used in molecular biology as it adds to the solubility of the proteins expressed in ''E. coli'' by being fused to them. The MBP-fusion protein can be purified by binding it to an amylose column followed by elution with maltose. | ||
==3D structures of maltose-binding protein== | ==3D structures of maltose-binding protein== | ||
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**[[1zjl]] – MBP (mutant) + Zn<br /> | **[[1zjl]] – MBP (mutant) + Zn<br /> | ||
**[[1zmg]] - MBP (mutant) + Cu<br /> | **[[1zmg]] - MBP (mutant) + Cu<br /> | ||
**[[4log]] - MBP /nuclear receptor<br /> | |||
**[[4ogm]] - MBP /pilin<br /> | |||
**[[4pe2]] - MBP /pilA1<br /> | |||
**[[4khz]] - MBP MalK, MalF, MalG, MBP <br /> | |||
**[[3fh6]] - MBP MalK, MalF, MalG <br /> | |||
**[[2xd2]] – SpMBP – ''Streptococcus pneumoniae'' | **[[2xd2]] – SpMBP – ''Streptococcus pneumoniae'' | ||
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**[[1n3w]], [[1nl5]], [[1mpd]], [[1iud]], [[1jvx]], [[1jvy]] - MBP (mutant) + maltose<br /> | **[[1n3w]], [[1nl5]], [[1mpd]], [[1iud]], [[1jvx]], [[1jvy]] - MBP (mutant) + maltose<br /> | ||
**[[1anf]], [[1jw5]] - MBP + maltose<br /> | **[[1anf]], [[1jw5]], [[2xz3]] - MBP + maltose<br /> | ||
**[[3mbp]] - MBP + maltotriose<br /> | **[[3mbp]] - MBP + maltotriose<br /> | ||
**[[1ez9]] - MBP + maltodextrin<br /> | **[[1ez9]] - MBP + maltodextrin<br /> | ||
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**[[2h25]] - MBP + β-cyclodextrin<br /> | **[[2h25]] - MBP + β-cyclodextrin<br /> | ||
**[[1fqa]], [[1fqb]], [[1fqc]], [[1fqd]] - MBP + maltotetraitol<br /> | **[[1fqa]], [[1fqb]], [[1fqc]], [[1fqd]] - MBP + maltotetraitol<br /> | ||
**[[3puy]], [[3puz]] - MBP MalK, MalF, MalG, MBP + maltose + AMPPNP<br /> | |||
**[[3rlf]] - MBP MalK, MalF, MalG, MBP + maltose + AMPPNP<br /> | |||
**[[4jbw]] - MBP MalK, MalF, MalG + EIIAGLC<br /> | |||
**[[3pv0]] - MBP MalK, MalF, MalG, MBP + maltose <br /> | |||
**[[2r6g]], [[3puv]], [[3puw]], [[3pux]], [[4ki0]] - MBP MalK, MalF, MalG, MBP + maltose + nucleotide<br /> | |||
**[[3ob4]] - MBP AraH2 (mutant) + maltotriose<br /> | |||
**[[1elj]] – MBP + glucose – ''Pyrococcus furiosus''<br /> | **[[1elj]] – MBP + glucose – ''Pyrococcus furiosus''<br /> | ||
**[[3jyr]] – MBP + α-acarbose – ''Salmonella enterica''<br /> | **[[3jyr]] – MBP + α-acarbose – ''Salmonella enterica''<br /> | ||
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**[[1t0k]] - MBP + ribosomal protein L30 + RNA<br /> | **[[1t0k]] - MBP + ribosomal protein L30 + RNA<br /> | ||
**[[3rum]] – MBP + ristocetin<br /> | **[[3rum]] – MBP + ristocetin<br /> | ||
**[[4nuf]] - MBP /nuclear receptor + orphan nuclear receptor peptide<br /> | |||
}} | }} | ||
[[Category:Topic Page]] | [[Category:Topic Page]] | ||
Revision as of 13:37, 21 January 2015
Maltose-binding protein (MBP) or ABC maltose transporter is part of the maltose/maltodextrin system in E. coli which is responsible of the catabolism of maltodextrin. MBP binds maltodextrin when the latter diffuses into the periplasm and passes it to proteins involved in active transport. MBP is widely used in molecular biology as it adds to the solubility of the proteins expressed in E. coli by being fused to them. The MBP-fusion protein can be purified by binding it to an amylose column followed by elution with maltose.
3D structures of maltose-binding protein3D structures of maltose-binding protein
Updated on 21-January-2015