3vsz: Difference between revisions
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[[ | ==Crystal structure of Ct1,3Gal43A in complex with galactan== | ||
<StructureSection load='3vsz' size='340' side='right' caption='[[3vsz]], [[Resolution|resolution]] 2.89Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3vsz]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_thermocellum_atcc_27405 Clostridium thermocellum atcc 27405]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VSZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3VSZ FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene></td></tr> | |||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3vsf|3vsf]], [[3vt0|3vt0]], [[3vt1|3vt1]], [[3vt2|3vt2]]</td></tr> | |||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Cthe_0661 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=203119 Clostridium thermocellum ATCC 27405])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vsz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vsz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3vsz RCSB], [http://www.ebi.ac.uk/pdbsum/3vsz PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Glycoside hydrolase family 43 (GH43) consists of a variety of enzymes distributed widely in prokaryotes and eukaryotes. The mechanism by which GH43 enzymes hydrolyze oligosaccharides requires three essential acidic amino acid residues. However, one of them is thought to be missing in galactan beta-1,3-galactosidases from the GH43 family. Ct1,3Gal43A, from Clostridium thermocellum, is comprised of a GH43 domain, a CBM13 domain, and a dockerin domain and exhibits an unusual ability to hydrolyze beta-1,3-galactan in the presence of a beta-1,6 linked branch. Here, we present its crystal structure at 2.7 A resolution and complex structures of the enzyme with several substrates and analogs. Two modes of substrate binding were observed at the beta site of the CtCBM13 domain, and one galactobiose molecule was found in an "L" shaped pocket of the CtGH43 domain, which appears large enough to accommodate two more galactose units. In addition, we found that mutating Glu112 to Gln or Ala eliminated the galactan hydrolysis activity of Ct1,3Gal43A while did not disrupt its ligand binding ability. Combining this results and the crystal structure we identified Glu112 in Ct1,3Gal43A as the 'missing' essential acidic residue in galactan beta-1,3-galactosidases. Structural information presented here also suggests a mechanism by which Ct1,3Gal43A bypasses beta-1,6 linked branches in the substrate and another mechanism by which the substrate is delivered 'in trans' from the CBM13 domain to the catalytic GH43 domain. | |||
Crystal structure of 1,3Gal43A, an exo-beta-1,3-galactanase from Clostridium thermocellum.,Jiang D, Fan J, Wang X, Zhao Y, Huang B, Liu J, Zhang XC J Struct Biol. 2012 Dec;180(3):447-57. doi: 10.1016/j.jsb.2012.08.005. Epub 2012 , Aug 29. PMID:22960181<ref>PMID:22960181</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
==See Also== | |||
*[[Ricin|Ricin]] | |||
== | == References == | ||
[[ | <references/> | ||
__TOC__ | |||
</StructureSection> | |||
[[Category: Clostridium thermocellum atcc 27405]] | [[Category: Clostridium thermocellum atcc 27405]] | ||
[[Category: Fan, J | [[Category: Fan, J]] | ||
[[Category: Huang, B | [[Category: Huang, B]] | ||
[[Category: Jiang, D | [[Category: Jiang, D]] | ||
[[Category: Wang, X | [[Category: Wang, X]] | ||
[[Category: Zhang, X C | [[Category: Zhang, X C]] | ||
[[Category: Zhao, Y | [[Category: Zhao, Y]] | ||
[[Category: Cbm13]] | [[Category: Cbm13]] | ||
[[Category: Galactan hydrolysis]] | [[Category: Galactan hydrolysis]] | ||
[[Category: Gh43]] | [[Category: Gh43]] | ||
[[Category: Sugar binding protein]] | [[Category: Sugar binding protein]] | ||
Revision as of 13:45, 4 January 2015
Crystal structure of Ct1,3Gal43A in complex with galactanCrystal structure of Ct1,3Gal43A in complex with galactan
Structural highlights
Publication Abstract from PubMedGlycoside hydrolase family 43 (GH43) consists of a variety of enzymes distributed widely in prokaryotes and eukaryotes. The mechanism by which GH43 enzymes hydrolyze oligosaccharides requires three essential acidic amino acid residues. However, one of them is thought to be missing in galactan beta-1,3-galactosidases from the GH43 family. Ct1,3Gal43A, from Clostridium thermocellum, is comprised of a GH43 domain, a CBM13 domain, and a dockerin domain and exhibits an unusual ability to hydrolyze beta-1,3-galactan in the presence of a beta-1,6 linked branch. Here, we present its crystal structure at 2.7 A resolution and complex structures of the enzyme with several substrates and analogs. Two modes of substrate binding were observed at the beta site of the CtCBM13 domain, and one galactobiose molecule was found in an "L" shaped pocket of the CtGH43 domain, which appears large enough to accommodate two more galactose units. In addition, we found that mutating Glu112 to Gln or Ala eliminated the galactan hydrolysis activity of Ct1,3Gal43A while did not disrupt its ligand binding ability. Combining this results and the crystal structure we identified Glu112 in Ct1,3Gal43A as the 'missing' essential acidic residue in galactan beta-1,3-galactosidases. Structural information presented here also suggests a mechanism by which Ct1,3Gal43A bypasses beta-1,6 linked branches in the substrate and another mechanism by which the substrate is delivered 'in trans' from the CBM13 domain to the catalytic GH43 domain. Crystal structure of 1,3Gal43A, an exo-beta-1,3-galactanase from Clostridium thermocellum.,Jiang D, Fan J, Wang X, Zhao Y, Huang B, Liu J, Zhang XC J Struct Biol. 2012 Dec;180(3):447-57. doi: 10.1016/j.jsb.2012.08.005. Epub 2012 , Aug 29. PMID:22960181[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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