1ue5: Difference between revisions
No edit summary |
No edit summary |
||
| Line 3: | Line 3: | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[1ue5]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UE5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1UE5 FirstGlance]. <br> | <table><tr><td colspan='2'>[[1ue5]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UE5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1UE5 FirstGlance]. <br> | ||
</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>< | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene></td></tr> | ||
<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1ue1|1ue1]], [[1ue6|1ue6]], [[1ue7|1ue7]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1ue1|1ue1]], [[1ue6|1ue6]], [[1ue7|1ue7]]</td></tr> | ||
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ue5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ue5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ue5 RCSB], [http://www.ebi.ac.uk/pdbsum/1ue5 PDBsum], [http://www.topsan.org/Proteins/TBSGC/1ue5 TOPSAN]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ue5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ue5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ue5 RCSB], [http://www.ebi.ac.uk/pdbsum/1ue5 PDBsum], [http://www.topsan.org/Proteins/TBSGC/1ue5 TOPSAN]</span></td></tr> | ||
<table> | </table> | ||
== Function == | |||
[[http://www.uniprot.org/uniprot/SSB_MYCTU SSB_MYCTU]] This protein is essential for replication of the chromosome. It is also involved in DNA recombination and repair (By similarity). | |||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
| Line 33: | Line 35: | ||
</StructureSection> | </StructureSection> | ||
[[Category: Mycobacterium tuberculosis]] | [[Category: Mycobacterium tuberculosis]] | ||
[[Category: Acharya, N | [[Category: Acharya, N]] | ||
[[Category: Jeyakanthan, J | [[Category: Jeyakanthan, J]] | ||
[[Category: Saikrishnan, K | [[Category: Saikrishnan, K]] | ||
[[Category: Sekar, K | [[Category: Sekar, K]] | ||
[[Category: | [[Category: Structural genomic]] | ||
[[Category: Varshney, U | [[Category: Varshney, U]] | ||
[[Category: Venkatesh, J | [[Category: Venkatesh, J]] | ||
[[Category: Vijayan, M | [[Category: Vijayan, M]] | ||
[[Category: Dna binding protein]] | [[Category: Dna binding protein]] | ||
[[Category: Dna-binding protein]] | [[Category: Dna-binding protein]] | ||
[[Category: Oligonucleotide binding fold]] | [[Category: Oligonucleotide binding fold]] | ||
[[Category: Protein structure initiative | [[Category: PSI, Protein structure initiative]] | ||
[[Category: Tbsgc]] | [[Category: Tbsgc]] | ||
Revision as of 23:51, 25 December 2014
Crystal structure of the single-stranded dna-binding protein from mycobacterium tuberculosisCrystal structure of the single-stranded dna-binding protein from mycobacterium tuberculosis
Structural highlights
Function[SSB_MYCTU] This protein is essential for replication of the chromosome. It is also involved in DNA recombination and repair (By similarity). Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedSingle-stranded DNA-binding protein (SSB) is an essential protein necessary for the functioning of the DNA replication, repair and recombination machineries. Here we report the structure of the DNA-binding domain of Mycobacterium tuberculosis SSB (MtuSSB) in four different crystals distributed in two forms. The structure of one of the forms was solved by a combination of isomorphous replacement and anomalous scattering. This structure was used to determine the structure of the other form by molecular replacement. The polypeptide chain in the structure exhibits the oligonucleotide binding fold. The globular core of the molecule in different subunits in the two forms and those in Escherichia coli SSB (EcoSSB) and human mitochondrial SSB (HMtSSB) have similar structure, although the three loops exhibit considerable structural variation. However, the tetrameric MtuSSB has an as yet unobserved quaternary association. This quaternary structure with a unique dimeric interface lends the oligomeric protein greater stability, which may be of significance to the functioning of the protein under conditions of stress. Also, as a result of the variation in the quaternary structure the path adopted by the DNA to wrap around MtuSSB is expected to be different from that of EcoSSB. Structure of Mycobacterium tuberculosis single-stranded DNA-binding protein. Variability in quaternary structure and its implications.,Saikrishnan K, Jeyakanthan J, Venkatesh J, Acharya N, Sekar K, Varshney U, Vijayan M J Mol Biol. 2003 Aug 8;331(2):385-93. PMID:12888346[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences |
| ||||||||||||||||||