Proteopedia

Single-stranded DNA-binding protein


Single-stranded DNA-binding protein (SSB) binds to single-stranded regions of DNA (ssDNA) to protect it from annealing, prevention of secondary structure formation and digestion. SSB binds to DNA in a sequence-independent manner. The binding of SSB enables processes like replication, DNA repair and recombination to occur. SSB can form complexes with specific genome maintenance proteins helping their activities[1].

  • Viral SSB proteins are monomeric.
  • Eukaryotic SSB (Replication protein A – RPA) is a heterotrimer composed of RPA70, RPA32 and RPA14. RPA has 6 DNA-binding domains (DBD).
  • Replication protein A70 is the eukaryotic SSB 70kDa subunit[2].
  • Mitochondrial human SSB (HsmtSSB) is a homotetramer.

See also Single stranded binding protein.

3D Structures of single-stranded DNA-binding protein

Single-stranded DNA-binding protein 3D structures


E. coli SSB complex with ssDNA (stick model) and UMP 1eqq

Drag the structure with the mouse to rotate

ReferencesReferences

  1. ↑ Marceau AH. Functions of single-strand DNA-binding proteins in DNA replication, recombination, and repair. Methods Mol Biol. 2012;922:1-21. doi: 10.1007/978-1-62703-032-8_1. PMID:22976174 doi:http://dx.doi.org/10.1007/978-1-62703-032-8_1
  2. ↑ Ishibashi T, Kimura S, Furukawa T, Hatanaka M, Hashimoto J, Sakaguchi K. Two types of replication protein A 70 kDa subunit in rice, Oryza sativa: molecular cloning, characterization, and cellular & tissue distribution. Gene. 2001 Jul 11;272(1-2):335-43. PMID:11470540 doi:10.1016/s0378-1119(01)00555-8

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