4o0a: Difference between revisions

From Proteopedia
Jump to navigation Jump to search
← Older editNewer edit →
No edit summary
No edit summary
Line 2: Line 2:
<StructureSection load='4o0a' size='340' side='right' caption='[[4o0a]], [[Resolution|resolution]] 1.20&Aring;' scene=''>
<StructureSection load='4o0a' size='340' side='right' caption='[[4o0a]], [[Resolution|resolution]] 1.20&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[4o0a]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4O0A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4O0A FirstGlance]. <br>
<table><tr><td colspan='2'>[[4o0a]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4O0A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4O0A FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=2P9:5-{4-[({[4-(5-CARBOXYFURAN-2-YL)-2-CHLOROPHENYL]CARBONOTHIOYL}AMINO)METHYL]PHENYL}-1-(3,4-DICHLOROPHENYL)-1H-PYRAZOLE-3-CARBOXYLIC+ACID'>2P9</scene></td></tr>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=2P9:5-{4-[({[4-(5-CARBOXYFURAN-2-YL)-2-CHLOROPHENYL]CARBONOTHIOYL}AMINO)METHYL]PHENYL}-1-(3,4-DICHLOROPHENYL)-1H-PYRAZOLE-3-CARBOXYLIC+ACID'>2P9</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4luv|4luv]], [[4lwc|4lwc]], [[4lwi|4lwi]], [[4luz|4luz]], [[4luo|4luo]]</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4luv|4luv]], [[4lwc|4lwc]], [[4lwi|4lwi]], [[4luz|4luz]], [[4luo|4luo]]</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">RPA1, REPA1, RPA70 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4o0a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4o0a OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4o0a RCSB], [http://www.ebi.ac.uk/pdbsum/4o0a PDBsum]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4o0a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4o0a OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4o0a RCSB], [http://www.ebi.ac.uk/pdbsum/4o0a PDBsum]</span></td></tr>
</table>
</table>
== Function ==
[[http://www.uniprot.org/uniprot/RFA1_HUMAN RFA1_HUMAN]] Plays an essential role in several cellular processes in DNA metabolism including replication, recombination and DNA repair. Binds and subsequently stabilizes single-stranded DNA intermediates and thus prevents complementary DNA from reannealing.<ref>PMID:19116208</ref> <ref>PMID:19996105</ref>  Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.<ref>PMID:19116208</ref> <ref>PMID:19996105</ref> 


==See Also==
==See Also==
*[[Single-stranded DNA-binding protein|Single-stranded DNA-binding protein]]
*[[Single-stranded DNA-binding protein|Single-stranded DNA-binding protein]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Camper, D V.]]
[[Category: Human]]
[[Category: Chazin, W J.]]
[[Category: Camper, D V]]
[[Category: Feldkamp, M D.]]
[[Category: Chazin, W J]]
[[Category: Fesik, S W.]]
[[Category: Feldkamp, M D]]
[[Category: Frank, A O.]]
[[Category: Fesik, S W]]
[[Category: Kennedy, J P.]]
[[Category: Frank, A O]]
[[Category: Olejniczak, E T.]]
[[Category: Kennedy, J P]]
[[Category: Patrone, J D.]]
[[Category: Olejniczak, E T]]
[[Category: Pelz, N F.]]
[[Category: Patrone, J D]]
[[Category: Rossanese, O W.]]
[[Category: Pelz, N F]]
[[Category: Vangamudi, B.]]
[[Category: Rossanese, O W]]
[[Category: Waterson, A G.]]
[[Category: Vangamudi, B]]
[[Category: Waterson, A G]]
[[Category: Dna binding protein-inhibitor complex]]
[[Category: Dna binding protein-inhibitor complex]]
[[Category: Ob-fold]]
[[Category: Ob-fold]]
[[Category: Protein-protein interaction]]
[[Category: Protein-protein interaction]]

Revision as of 04:26, 25 December 2014

Fragment-Based Discovery of a Potent Inhibitor of Replication Protein A Protein-Protein InteractionsFragment-Based Discovery of a Potent Inhibitor of Replication Protein A Protein-Protein Interactions

Structural highlights

4o0a is a 1 chain structure with sequence from Human. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Gene:RPA1, REPA1, RPA70 (HUMAN)
Resources:FirstGlance, OCA, RCSB, PDBsum

Function

[RFA1_HUMAN] Plays an essential role in several cellular processes in DNA metabolism including replication, recombination and DNA repair. Binds and subsequently stabilizes single-stranded DNA intermediates and thus prevents complementary DNA from reannealing.[1] [2] Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.[3] [4]

See Also

References

  1. ↑ Mason AC, Haring SJ, Pryor JM, Staloch CA, Gan TF, Wold MS. An alternative form of replication protein a prevents viral replication in vitro. J Biol Chem. 2009 Feb 20;284(8):5324-31. doi: 10.1074/jbc.M808963200. Epub 2008, Dec 29. PMID:19116208 doi:10.1074/jbc.M808963200
  2. ↑ Kemp MG, Mason AC, Carreira A, Reardon JT, Haring SJ, Borgstahl GE, Kowalczykowski SC, Sancar A, Wold MS. An alternative form of replication protein a expressed in normal human tissues supports DNA repair. J Biol Chem. 2010 Feb 12;285(7):4788-97. doi: 10.1074/jbc.M109.079418. Epub 2009 , Dec 7. PMID:19996105 doi:10.1074/jbc.M109.079418
  3. ↑ Mason AC, Haring SJ, Pryor JM, Staloch CA, Gan TF, Wold MS. An alternative form of replication protein a prevents viral replication in vitro. J Biol Chem. 2009 Feb 20;284(8):5324-31. doi: 10.1074/jbc.M808963200. Epub 2008, Dec 29. PMID:19116208 doi:10.1074/jbc.M808963200
  4. ↑ Kemp MG, Mason AC, Carreira A, Reardon JT, Haring SJ, Borgstahl GE, Kowalczykowski SC, Sancar A, Wold MS. An alternative form of replication protein a expressed in normal human tissues supports DNA repair. J Biol Chem. 2010 Feb 12;285(7):4788-97. doi: 10.1074/jbc.M109.079418. Epub 2009 , Dec 7. PMID:19996105 doi:10.1074/jbc.M109.079418

4o0a, resolution 1.20Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA
Retrieved from "https://proteopedia.openfox.io/wiki/index.php?title=4o0a&oldid=2242835"