1a18: Difference between revisions
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== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[1a18]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A18 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1A18 FirstGlance]. <br> | <table><tr><td colspan='2'>[[1a18]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A18 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1A18 FirstGlance]. <br> | ||
</td></tr><tr><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NPH:CYSTEINE-METHYLENE-CARBAMOYL-1,10-PHENANTHROLINE'>NPH</scene></td></tr> | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NPH:CYSTEINE-METHYLENE-CARBAMOYL-1,10-PHENANTHROLINE'>NPH</scene></td></tr> | ||
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1a18 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a18 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1a18 RCSB], [http://www.ebi.ac.uk/pdbsum/1a18 PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1a18 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a18 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1a18 RCSB], [http://www.ebi.ac.uk/pdbsum/1a18 PDBsum]</span></td></tr> | ||
<table> | </table> | ||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
[[Category: Banaszak, L | [[Category: Banaszak, L]] | ||
[[Category: Davies, R | [[Category: Davies, R]] | ||
[[Category: Distefano, M | [[Category: Distefano, M]] | ||
[[Category: Kuang, H | [[Category: Kuang, H]] | ||
[[Category: Mazhary, A | [[Category: Mazhary, A]] | ||
[[Category: Ory, J | [[Category: Ory, J]] | ||
[[Category: Fatty acid binding protein]] | [[Category: Fatty acid binding protein]] | ||
[[Category: Phosphorylation]] | [[Category: Phosphorylation]] | ||
[[Category: Transport]] | [[Category: Transport]] | ||
Revision as of 12:45, 22 December 2014
PHENANTHROLINE MODIFIED MURINE ADIPOCYTE LIPID BINDING PROTEINPHENANTHROLINE MODIFIED MURINE ADIPOCYTE LIPID BINDING PROTEIN
Structural highlights
Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedAdipocyte lipid-binding protein (ALBP) is a small (14.5 kDa) 10-stranded beta-barrel protein found in mammalian fat cells. The crystal structures of various holo-forms of ALBP have been solved and show the fatty acid ligand bound in a large (approximately 400 A3) cavity isolated from bulk solvent. Examination of the cavity suggests that it would be a good site for the creation of an artificial catalyst, as numerous well defined crystal structures of ALBP are available and past studies have shown the conformation to be reasonably tolerant to modification and mutagenesis. Previous work has shown ALBP to be a good protein scaffold for exploring enantio- and stereoselective reactions; two constructs, ALBP attached to either a pyridoxamine or a phenanthroline group at C117, have been chemically characterized. Both modified proteins have been crystallized and their structures solved and refined. The X-ray models have been used to examine the origin of the chiral selectivity seen in the products. It is apparent that these covalent adducts reduce the internal cavity volume, sterically limiting substrate interactions with the reactive groups, as well as solvent access to potential intermediates in the reaction pathway. Structural characterization of two synthetic catalysts based on adipocyte lipid-binding protein.,Ory JJ, Mazhary A, Kuang H, Davies RR, Distefano MD, Banaszak LJ Protein Eng. 1998 Apr;11(4):253-61. PMID:9680187[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences |
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