4jo6: Difference between revisions

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-{{STRUCTURE_4jo6|  PDB=4jo6  |  SCENE=  }}
+==Streptavidin complex with SBP-Tag==
-===Streptavidin complex with SBP-Tag===
+<StructureSection load='4jo6' size='340' side='right' caption='[[4jo6]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
-{{ABSTRACT_PUBMED_23633599}}
+== Structural highlights ==
+<table><tr><td colspan='2'>[[4jo6]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JO6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4JO6 FirstGlance]. <br>
+</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1swe|1swe]]</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4jo6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4jo6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4jo6 RCSB], [http://www.ebi.ac.uk/pdbsum/4jo6 PDBsum]</span></td></tr>
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The 38-residue SBP-Tag binds to streptavidin more tightly (K(d) -/= 2.5-4.9 nM) than most if not all other known peptide sequences. Crystallographic analysis at 1.75 A resolution shows that the SBP-Tag binds to streptavidin in an unprecedented manner by simultaneously interacting with biotin-binding pockets from two separate subunits. An N-terminal HVV peptide sequence (residues 12-14) and a C-terminal HPQ sequence (residues 31-33) form the bulk of the direct interactions between the SBP-Tag and the two biotin-binding pockets. Surprisingly, most of the peptide spanning these two sites (residues 17-28) adopts a regular alpha-helical structure that projects three leucine side chains into a groove formed at the interface between two streptavidin protomers. The crystal structure shows that residues 1-10 and 35-38 of the original SBP-Tag identified through in vitro selection and deletion analysis do not appear to contact streptavidin and thus may not be important for binding. A 25-residue peptide comprising residues 11-34 (SBP-Tag2) was synthesized and shown using surface plasmon resonance to bind streptavidin with very similar affinity and kinetics when compared with the SBP-Tag. The SBP-Tag2 was also added to the C-terminus of beta-lactamase and was shown to be just as effective as the full-length SBP-Tag in affinity purification. These results validate the molecular structure of the SBP-Tag-streptavidin complex and establish a minimal bivalent streptavidin-binding tag from which further rational design and optimization can proceed.
-==Function==
+The structure of the SBP-Tag-streptavidin complex reveals a novel helical scaffold bridging binding pockets on separate subunits.,Barrette-Ng IH, Wu SC, Tjia WM, Wong SL, Ng KK Acta Crystallogr D Biol Crystallogr. 2013 May;69(Pt 5):879-87. doi:, 10.1107/S0907444913002576. Epub 2013 Apr 19. PMID:23633599<ref>PMID:23633599</ref>
-[[http://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV]] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).
-==About this Structure==
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[4jo6]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JO6 OCA].
+</div>
-==Reference==
+==See Also==
-<ref group="xtra">PMID:023633599</ref><references group="xtra"/><references/>
+*[[Avidin|Avidin]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
 [[Category: Streptomyces avidinii]]
-[[Category: Barrette-Ng, I H.]]
+[[Category: Barrette-Ng, I H]]
-[[Category: Ng, K K.S.]]
+[[Category: Ng, K K.S]]
-[[Category: Tjia, W M.]]
+[[Category: Tjia, W M]]
-[[Category: Wong, S L.]]
+[[Category: Wong, S L]]
-[[Category: Wu, S C.]]
+[[Category: Wu, S C]]
 [[Category: Biotechnological targeting]]
 [[Category: Biotin and peptide binding]]
 [[Category: Homotetramer bound to peptide]]
 [[Category: Unknown function]]