Transposase

Function Transposases bind to the ends of a transposon and catalyze its movement to another part of the genome by a cut-and-paste mechanism. Transposase contains a DDE motif in its active site enabling it to coordinate the binding of divalent metal ions. The metal ions affect transposase reactivity. Mu transposase (MuA) is essential for integration, replication-transposition and excision of bacteriophage Mu DNA into multiple sites of bacterial genome. Mu transposition occurs within the transposome which is a protein-DNA complex which includes 4 subunits of MuA.

spinqualitylabelsall models Structure of Mu transposase complex with DNA (PDB code 4fcy). Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

3D structures of transposase3D structures of transposase

Updated on 15-December-2014

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• Mu transposase
  • 1tns, 1tnt – MuMuA DNA-binding domain – enterobacteria phage Mu - NMR
    
  • 1bcm, 1bco – MuMuA core domain
    
  • 2ezh, 2ezi – MuMuA Iγ subdomain - NMR
    
  • 2ezl – MuMuA Iβ subdomain - NMR
    
  • 4fcy – MuMuA + DNA
    
• Hermes transposase
  • 2bw3 – fTra (mutant) - fly
    
  • 4d1q – fTra (mutant) + DNA
    
• Dra2 transposase
  • 2xm3 – Dra2 + Dra2 transposase binding element + DNA – Deinococcus radiodurans
    
  • 2xma, 2xqc – Dra2 + DNA
    
  • 2xo6 – Dra2 (mutant) + DNA
    
• Tn5 transposase
  • 1muh, 1mus, 1mm8, 3ecp, 4dm0 – EcTn5 (mutant) + DNA – Escherichia coli
    
• Mos1 transposase
  • 2f7t – DmMos1 catalytic domain (mutant) – Drosophila mauritiana
    
  • 3hos, 3hot – DmMos1 (mutant) + DNA
    
  • 4mda, 4mdb – DmMos1 catalytic domain (mutant) + drug
    
• Tc3 transposase
  • 1tc3, 1u78 – Tc3 DNA-binding domain (mutant) + DNA – Caenorhabditis elegans