1br5: Difference between revisions
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[[Image:1br5.gif|left|200px]] | [[Image:1br5.gif|left|200px]] | ||
'''RICIN A CHAIN (RECOMBINANT) COMPLEX WITH NEOPTERIN''' | {{Structure | ||
|PDB= 1br5 |SIZE=350|CAPTION= <scene name='initialview01'>1br5</scene>, resolution 2.5Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=NEO:NEOPTERIN'>NEO</scene> | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/rRNA_N-glycosylase rRNA N-glycosylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.22 3.2.2.22] | |||
|GENE= | |||
}} | |||
'''RICIN A CHAIN (RECOMBINANT) COMPLEX WITH NEOPTERIN''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1BR5 is a [ | 1BR5 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Ricinus_communis Ricinus communis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BR5 OCA]. | ||
==Reference== | ==Reference== | ||
Structure-based identification of a ricin inhibitor., Yan X, Hollis T, Svinth M, Day P, Monzingo AF, Milne GW, Robertus JD, J Mol Biol. 1997 Mar 14;266(5):1043-9. PMID:[http:// | Structure-based identification of a ricin inhibitor., Yan X, Hollis T, Svinth M, Day P, Monzingo AF, Milne GW, Robertus JD, J Mol Biol. 1997 Mar 14;266(5):1043-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9086280 9086280] | ||
[[Category: Ricinus communis]] | [[Category: Ricinus communis]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: glycosidase]] | [[Category: glycosidase]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:14:38 2008'' | ||
Revision as of 11:14, 20 March 2008
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| , resolution 2.5Å | |||||||
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| Ligands: | |||||||
| Activity: | rRNA N-glycosylase, with EC number 3.2.2.22 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
RICIN A CHAIN (RECOMBINANT) COMPLEX WITH NEOPTERIN
OverviewOverview
Ricin is a potent cytotoxin which has been used widely in the construction of therapeutic agents such as immunotoxins. Recently it has been used by governments and underground groups as a poison. There is interest in identifying and designing effective inhibitors of the ricin A chain (RTA). In this study computer-assisted searches indicated that pterins might bind in the RTA active site which normally recognizes a specific adenine base on rRNA. Kinetic assays showed that pteroic acid could inhibit RTA activity with an apparent Ki of 0.6 mM. A 2.3 A crystal structure of the complex revealed the mode of binding. The pterin ring displaces Tyr80 and binds in the adenine pocket making specific hydrogen bonds to active site residues. The benzoate moiety of pteroic acid binds on the opposite side of Tyr80 making van der Waals contact with the Tyr ring and forming a hydrogen bond with Asn78. Neopterin, a propane triol derivative of pterin, also binds to RTA as revealed by the X-ray structure of its complex with RTA. Neither pterin-6-carboxylic acid nor folic acid bind to the crystal or act as inhibitors. The models observed suggest alterations to the pterin moiety which may produce more potent and specific RTA inhibitors.
About this StructureAbout this Structure
1BR5 is a Single protein structure of sequence from Ricinus communis. Full crystallographic information is available from OCA.
ReferenceReference
Structure-based identification of a ricin inhibitor., Yan X, Hollis T, Svinth M, Day P, Monzingo AF, Milne GW, Robertus JD, J Mol Biol. 1997 Mar 14;266(5):1043-9. PMID:9086280
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