1acf: Difference between revisions

Revision as of 10:55, 20 March 2008

, resolution 2.0Å

Coordinates:

ACANTHAMOEBA CASTELLANII PROFILIN IB

OverviewOverview

We determined the structures of Acanthamoeba profilin I and profilin II by x-ray crystallography at resolutions of 2.0 and 2.8 A, respectively. The polypeptide folds and the actin-binding surfaces of the amoeba profilins are very similar to those of bovine and human profilins. The electrostatic potential surfaces of the two Acanthamoeba isoforms differ. Two areas of high positive potential on the surface of profilin II are candidate binding sites for phosphatidylinositol phosphates. The proximity of these sites to the actin binding site provides an explanation for the competition between actin and lipids for binding profilin.

About this StructureAbout this Structure

1ACF is a Single protein structure of sequence from Acanthamoeba castellanii. Full crystallographic information is available from OCA.

ReferenceReference

X-ray structures of isoforms of the actin-binding protein profilin that differ in their affinity for phosphatidylinositol phosphates., Fedorov AA, Magnus KA, Graupe MH, Lattman EE, Pollard TD, Almo SC, Proc Natl Acad Sci U S A. 1994 Aug 30;91(18):8636-40. PMID:8078936

Page seeded by OCA on Thu Mar 20 09:55:42 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1acf.gif|left|200px]]<br /><applet load="1acf" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1acf.gif|left|200px]]
-caption="1acf, resolution 2.0&Aring;" />
-'''ACANTHAMOEBA CASTELLANII PROFILIN IB'''<br />
+ {{Structure
+|PDB= 1acf |SIZE=350|CAPTION= <scene name='initialview01'>1acf</scene>, resolution 2.0&Aring;
+|SITE=
+|LIGAND=
+|ACTIVITY=
+|GENE=
+}}
+'''ACANTHAMOEBA CASTELLANII PROFILIN IB'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-ACF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Acanthamoeba_castellanii Acanthamoeba castellanii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ACF OCA].
+ACF is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Acanthamoeba_castellanii Acanthamoeba castellanii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ACF OCA].
 ==Reference==
-X-ray structures of isoforms of the actin-binding protein profilin that differ in their affinity for phosphatidylinositol phosphates., Fedorov AA, Magnus KA, Graupe MH, Lattman EE, Pollard TD, Almo SC, Proc Natl Acad Sci U S A. 1994 Aug 30;91(18):8636-40. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8078936 8078936]
+X-ray structures of isoforms of the actin-binding protein profilin that differ in their affinity for phosphatidylinositol phosphates., Fedorov AA, Magnus KA, Graupe MH, Lattman EE, Pollard TD, Almo SC, Proc Natl Acad Sci U S A. 1994 Aug 30;91(18):8636-40. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8078936 8078936]
 [[Category: Acanthamoeba castellanii]]
 [[Category: Single protein]]
@@ Line 23: / Line 32: @@
 [[Category: protein binding]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:43:07 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 09:55:42 2008''