2bjv: Difference between revisions

Revision as of 17:38, 21 February 2008

CRYSTAL STRUCTURE OF PSPF(1-275) R168A MUTANT

OverviewOverview

Activators of bacterial sigma54-RNA polymerase holoenzyme are mechanochemical proteins that use adenosine triphosphate (ATP) hydrolysis to activate transcription. We have determined by cryogenic electron microscopy (cryo-EM) a 20 angstrom resolution structure of an activator, phage shock protein F [PspF(1-275)], which is bound to an ATP transition state analog in complex with its basal factor, sigma54. By fitting the crystal structure of PspF(1-275) at 1.75 angstroms into the EM map, we identified two loops involved in binding sigma54. Comparing enhancer-binding structures in different nucleotide states and mutational analysis led us to propose nucleotide-dependent conformational changes that free the loops for association with sigma54.

About this StructureAbout this Structure

2BJV is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

ReferenceReference

Structural insights into the activity of enhancer-binding proteins., Rappas M, Schumacher J, Beuron F, Niwa H, Bordes P, Wigneshweraraj S, Keetch CA, Robinson CV, Buck M, Zhang X, Science. 2005 Mar 25;307(5717):1972-5. PMID:15790859

Page seeded by OCA on Thu Feb 21 16:38:33 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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-[[Image:2bjv.gif|left|200px]]<br /><applet load="2bjv" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:2bjv.gif|left|200px]]<br /><applet load="2bjv" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="2bjv, resolution 1.70&Aring;" />
 '''CRYSTAL STRUCTURE OF PSPF(1-275) R168A MUTANT'''<br />
 ==Overview==
-Activators of bacterial sigma54-RNA polymerase holoenzyme are, mechanochemical proteins that use adenosine triphosphate (ATP) hydrolysis, to activate transcription. We have determined by cryogenic electron, microscopy (cryo-EM) a 20 angstrom resolution structure of an activator, phage shock protein F [PspF(1-275)], which is bound to an ATP transition, state analog in complex with its basal factor, sigma54. By fitting the, crystal structure of PspF(1-275) at 1.75 angstroms into the EM map, we, identified two loops involved in binding sigma54. Comparing, enhancer-binding structures in different nucleotide states and mutational, analysis led us to propose nucleotide-dependent conformational changes, that free the loops for association with sigma54.
+Activators of bacterial sigma54-RNA polymerase holoenzyme are mechanochemical proteins that use adenosine triphosphate (ATP) hydrolysis to activate transcription. We have determined by cryogenic electron microscopy (cryo-EM) a 20 angstrom resolution structure of an activator, phage shock protein F [PspF(1-275)], which is bound to an ATP transition state analog in complex with its basal factor, sigma54. By fitting the crystal structure of PspF(1-275) at 1.75 angstroms into the EM map, we identified two loops involved in binding sigma54. Comparing enhancer-binding structures in different nucleotide states and mutational analysis led us to propose nucleotide-dependent conformational changes that free the loops for association with sigma54.
 ==About this Structure==
-BJV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BJV OCA].
+BJV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BJV OCA].
 ==Reference==
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 [[Category: Bordes, P.]]
 [[Category: Buck, M.]]
-[[Category: Keetch, C.A.]]
+[[Category: Keetch, C A.]]
 [[Category: Niwa, H.]]
 [[Category: Rappas, M.]]
-[[Category: Robinson, C.V.]]
+[[Category: Robinson, C V.]]
 [[Category: Schumacher, J.]]
 [[Category: Wigneshweraraj, S.]]
@@ Line 33: / Line 33: @@
 [[Category: transcription regulation]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 08:48:29 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:38:33 2008''