1qqv: Difference between revisions

Revision as of 15:42, 21 February 2008

SOLUTION STRUCTURE OF THE HEADPIECE DOMAIN OF CHICKEN VILLIN

OverviewOverview

A growing family of F-actin-bundling proteins harbors a modular F-actin-binding headpiece domain at the C terminus. Headpiece provides one of the two F-actin-binding sites essential for filament bundling. Here, we report the first structure of a functional headpiece domain. The NMR structure of chicken villin headpiece (HP67) reveals two subdomains that share a tightly packed hydrophobic core. The N-terminal subdomain contains bends, turns, and a four-residue alpha-helix as well as a buried histidine residue that imparts a pH-dependent folding. The C-terminal subdomain is composed of three alpha-helices and its folding is pH-independent. Two residues previously implicated in F-actin-binding form a buried salt-bridge between the N and C-terminal subdomains. The rest of the identified actin-binding residues are solvent-exposed and map onto a unique F-actin-binding surface.

About this StructureAbout this Structure

1QQV is a Single protein structure of sequence from Gallus gallus. Full crystallographic information is available from OCA.

ReferenceReference

NMR structure of an F-actin-binding "headpiece" motif from villin., Vardar D, Buckley DA, Frank BS, McKnight CJ, J Mol Biol. 1999 Dec 17;294(5):1299-310. PMID:10600386

Page seeded by OCA on Thu Feb 21 14:42:41 2008

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OCA

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-[[Image:1qqv.gif|left|200px]]<br /><applet load="1qqv" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1qqv.gif|left|200px]]<br /><applet load="1qqv" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1qqv" />
 '''SOLUTION STRUCTURE OF THE HEADPIECE DOMAIN OF CHICKEN VILLIN'''<br />
 ==Overview==
-A growing family of F-actin-bundling proteins harbors a modular, F-actin-binding headpiece domain at the C terminus. Headpiece provides one, of the two F-actin-binding sites essential for filament bundling. Here, we, report the first structure of a functional headpiece domain. The NMR, structure of chicken villin headpiece (HP67) reveals two subdomains that, share a tightly packed hydrophobic core. The N-terminal subdomain contains, bends, turns, and a four-residue alpha-helix as well as a buried histidine, residue that imparts a pH-dependent folding. The C-terminal subdomain is, composed of three alpha-helices and its folding is pH-independent. Two, residues previously implicated in F-actin-binding form a buried, salt-bridge between the N and C-terminal subdomains. The rest of the, identified actin-binding residues are solvent-exposed and map onto a, unique F-actin-binding surface.
+A growing family of F-actin-bundling proteins harbors a modular F-actin-binding headpiece domain at the C terminus. Headpiece provides one of the two F-actin-binding sites essential for filament bundling. Here, we report the first structure of a functional headpiece domain. The NMR structure of chicken villin headpiece (HP67) reveals two subdomains that share a tightly packed hydrophobic core. The N-terminal subdomain contains bends, turns, and a four-residue alpha-helix as well as a buried histidine residue that imparts a pH-dependent folding. The C-terminal subdomain is composed of three alpha-helices and its folding is pH-independent. Two residues previously implicated in F-actin-binding form a buried salt-bridge between the N and C-terminal subdomains. The rest of the identified actin-binding residues are solvent-exposed and map onto a unique F-actin-binding surface.
 ==About this Structure==
-QQV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1QQV OCA].
+QQV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QQV OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Gallus gallus]]
 [[Category: Single protein]]
-[[Category: Buckley, D.A.]]
+[[Category: Buckley, D A.]]
-[[Category: Frank, B.S.]]
+[[Category: Frank, B S.]]
-[[Category: McKnight, C.J.]]
+[[Category: McKnight, C J.]]
 [[Category: Vardar, D.]]
 [[Category: f-actin binding domain]]
 [[Category: salt-bridge]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 00:54:19 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:42:41 2008''