1lta: Difference between revisions

Revision as of 14:48, 21 February 2008

2.2 ANGSTROMS CRYSTAL STRUCTURE OF E. COLI HEAT-LABILE ENTEROTOXIN (LT) WITH BOUND GALACTOSE

OverviewOverview

The galactose-binding site in cholera toxin and the closely related heat-labile enterotoxin (LT) from Escherichia coli is an attractive target for the rational design of potential anti-cholera drugs. In this paper we analyse the molecular structure of this binding site as seen in several crystal structures, including that of an LT:galactose complex which we report here at 2.2 A resolution. The binding surface on the free toxin contains several tightly associated water molecules and a relatively flexible loop consisting of residues 51-60 of the B subunit. During receptor binding this loop becomes tightly ordered by forming hydrogen bonds jointly to the GM1 pentasaccharide and to a set of water molecules which stabilize the toxin:receptor complex.

About this StructureAbout this Structure

1LTA is a Protein complex structure of sequences from Escherichia coli with as ligand. Full crystallographic information is available from OCA.

ReferenceReference

Galactose-binding site in Escherichia coli heat-labile enterotoxin (LT) and cholera toxin (CT)., Merritt EA, Sixma TK, Kalk KH, van Zanten BA, Hol WG, Mol Microbiol. 1994 Aug;13(4):745-53. PMID:7997185

Page seeded by OCA on Thu Feb 21 13:48:13 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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-[[Image:1lta.jpg|left|200px]]<br /><applet load="1lta" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1lta.jpg|left|200px]]<br /><applet load="1lta" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1lta, resolution 2.2&Aring;" />
 '''2.2 ANGSTROMS CRYSTAL STRUCTURE OF E. COLI HEAT-LABILE ENTEROTOXIN (LT) WITH BOUND GALACTOSE'''<br />
 ==Overview==
-The galactose-binding site in cholera toxin and the closely related, heat-labile enterotoxin (LT) from Escherichia coli is an attractive target, for the rational design of potential anti-cholera drugs. In this paper we, analyse the molecular structure of this binding site as seen in several, crystal structures, including that of an LT:galactose complex which we, report here at 2.2 A resolution. The binding surface on the free toxin, contains several tightly associated water molecules and a relatively, flexible loop consisting of residues 51-60 of the B subunit. During, receptor binding this loop becomes tightly ordered by forming hydrogen, bonds jointly to the GM1 pentasaccharide and to a set of water molecules, which stabilize the toxin:receptor complex.
+The galactose-binding site in cholera toxin and the closely related heat-labile enterotoxin (LT) from Escherichia coli is an attractive target for the rational design of potential anti-cholera drugs. In this paper we analyse the molecular structure of this binding site as seen in several crystal structures, including that of an LT:galactose complex which we report here at 2.2 A resolution. The binding surface on the free toxin contains several tightly associated water molecules and a relatively flexible loop consisting of residues 51-60 of the B subunit. During receptor binding this loop becomes tightly ordered by forming hydrogen bonds jointly to the GM1 pentasaccharide and to a set of water molecules which stabilize the toxin:receptor complex.
 ==About this Structure==
-LTA is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with GAL as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1LTA OCA].
+LTA is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=GAL:'>GAL</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LTA OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Escherichia coli]]
 [[Category: Protein complex]]
-[[Category: Hol, W.G.J.]]
+[[Category: Hol, W G.J.]]
-[[Category: Kalk, K.H.]]
+[[Category: Kalk, K H.]]
-[[Category: Merritt, E.A.]]
+[[Category: Merritt, E A.]]
-[[Category: Sixma, T.K.]]
+[[Category: Sixma, T K.]]
-[[Category: Zanten, B.A.M.Van.]]
+[[Category: Zanten, B A.M Van.]]
 [[Category: GAL]]
 [[Category: enterotoxin]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 20:51:49 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:48:13 2008''