1byo: Difference between revisions
No edit summary |
No edit summary |
||
| Line 4: | Line 4: | ||
==Overview== | ==Overview== | ||
Plastocyanin functions as an electron carrier between the cytochrome b6f | Plastocyanin functions as an electron carrier between the cytochrome b6f complex and photosystem I. The crystal structures of the wild-type and E43K/D44K double mutant from the higher plant, Silene, have been determined at 2.0 and 1.75 A resolution, respectively. The wild-type plastocyanin comprises two monomers per asymmetric unit, one of which shows the unusually great distance between the copper ion and the Ndelta1 atom of H87 because of the hydrogen bond network formation between H87 and symmetry-related G10. The root mean square deviation for Ca atoms between the wild-type and mutant plastocyanins is 0.44 A, however, the electrostatic potential maps of their molecular surfaces are remarkably different. The low electron-transfer rate in the E43K/D44K mutant results from the hindrance of electrostatic interactions, not from the structural change due to the mutation. | ||
==About this Structure== | ==About this Structure== | ||
| Line 10: | Line 10: | ||
==Reference== | ==Reference== | ||
Crystal structures of wild-type and mutant plastocyanins from a higher plant, Silene., Sugawara H, Inoue T, Li C, Gotowda M, Hibino T, Takabe T, Kai Y, J Biochem | Crystal structures of wild-type and mutant plastocyanins from a higher plant, Silene., Sugawara H, Inoue T, Li C, Gotowda M, Hibino T, Takabe T, Kai Y, J Biochem. 1999 May;125(5):899-903. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10220581 10220581] | ||
[[Category: Silene latifolia subsp. alba]] | [[Category: Silene latifolia subsp. alba]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 24: | Line 24: | ||
[[Category: photosynthesis]] | [[Category: photosynthesis]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:00:40 2008'' | ||
Revision as of 13:00, 21 February 2008
|
WILD-TYPE PLASTOCYANIN FROM SILENE
OverviewOverview
Plastocyanin functions as an electron carrier between the cytochrome b6f complex and photosystem I. The crystal structures of the wild-type and E43K/D44K double mutant from the higher plant, Silene, have been determined at 2.0 and 1.75 A resolution, respectively. The wild-type plastocyanin comprises two monomers per asymmetric unit, one of which shows the unusually great distance between the copper ion and the Ndelta1 atom of H87 because of the hydrogen bond network formation between H87 and symmetry-related G10. The root mean square deviation for Ca atoms between the wild-type and mutant plastocyanins is 0.44 A, however, the electrostatic potential maps of their molecular surfaces are remarkably different. The low electron-transfer rate in the E43K/D44K mutant results from the hindrance of electrostatic interactions, not from the structural change due to the mutation.
About this StructureAbout this Structure
1BYO is a Single protein structure of sequence from Silene latifolia subsp. alba with as ligand. Known structural/functional Sites: and . Full crystallographic information is available from OCA.
ReferenceReference
Crystal structures of wild-type and mutant plastocyanins from a higher plant, Silene., Sugawara H, Inoue T, Li C, Gotowda M, Hibino T, Takabe T, Kai Y, J Biochem. 1999 May;125(5):899-903. PMID:10220581
Page seeded by OCA on Thu Feb 21 12:00:40 2008