1byo

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WILD-TYPE PLASTOCYANIN FROM SILENEWILD-TYPE PLASTOCYANIN FROM SILENE

Structural highlights

1byo is a 2 chain structure with sequence from Silene latifolia subsp. alba. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

PLAS_SILLB Participates in electron transfer between P700 and the cytochrome b6-f complex in photosystem I.[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Plastocyanin functions as an electron carrier between the cytochrome b6f complex and photosystem I. The crystal structures of the wild-type and E43K/D44K double mutant from the higher plant, Silene, have been determined at 2.0 and 1.75 A resolution, respectively. The wild-type plastocyanin comprises two monomers per asymmetric unit, one of which shows the unusually great distance between the copper ion and the Ndelta1 atom of H87 because of the hydrogen bond network formation between H87 and symmetry-related G10. The root mean square deviation for Ca atoms between the wild-type and mutant plastocyanins is 0.44 A, however, the electrostatic potential maps of their molecular surfaces are remarkably different. The low electron-transfer rate in the E43K/D44K mutant results from the hindrance of electrostatic interactions, not from the structural change due to the mutation.

Crystal structures of wild-type and mutant plastocyanins from a higher plant, Silene.,Sugawara H, Inoue T, Li C, Gotowda M, Hibino T, Takabe T, Kai Y J Biochem. 1999 May;125(5):899-903. PMID:10220581[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

See Also

References

  1. Sugawara H, Inoue T, Li C, Gotowda M, Hibino T, Takabe T, Kai Y. Crystal structures of wild-type and mutant plastocyanins from a higher plant, Silene. J Biochem. 1999 May;125(5):899-903. PMID:10220581
  2. Sugawara H, Inoue T, Li C, Gotowda M, Hibino T, Takabe T, Kai Y. Crystal structures of wild-type and mutant plastocyanins from a higher plant, Silene. J Biochem. 1999 May;125(5):899-903. PMID:10220581

1byo, resolution 2.00Å

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