User:Mitchell Long/Sandbox 1: Difference between revisions

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Mobile Loop
Mobile Loop
(&#946;/&#945;)<SUP>8</SUP> Barrel
(&#946;/&#945;)<SUB>8</SUB> Barrel
Interface between Alpha and Beta Subunits
Interface between Alpha and Beta Subunits



Revision as of 06:27, 17 November 2011


Introduction

Luciferases are a class of enzymes that catalyze the oxidation of a long chain aliphatic aldehydes. This reaction results in the formation of a carboxylic acid and the emission of photons in the form of blue-green light.



Structure of Bacterial Luciferase and FMN complex from

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PDB ID 3fgc

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3fgc, resolution 2.30Å ()
Ligands: , ,
Gene: luxA (Vibrio harveyi), luxB (Vibrio harveyi)
Activity: Alkanal monooxygenase (FMN-linked), with EC number 1.14.14.3
Related: 1luc, 1brl
Resources: FirstGlance, OCA, RCSB, PDBsum
Coordinates: save as pdb, mmCIF, xml




Mechanism of Bio luminescenceMechanism of Bio luminescence

Luciferase found in 'Vibrio harveyi' is a heterodimer that is composed of a catalytic α subunit and a homologous but noncatalytic beta subunit. The catalytic


Structural MotifsStructural Motifs

Active Site

Mobile Loop (β/α)8 Barrel Interface between Alpha and Beta Subunits

Applications In BiotechnologyApplications In Biotechnology

The luciferase assay

Quorum SensingQuorum Sensing

Quorum sensing is a population density dependent form gene expression found in many bacteria.



ReferencesReferences