Tom Sandbox: Difference between revisions

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Alan Tom

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	The basic region binding domain of GCN4 causes a shift in bothe the AP-1 and ATF/CREB binding sites of DNA<ref name="bind">PMID:15459288</ref>. This was determined by adding fluorophores to the end of U shaped DNA segments with the specific binding sites in the centers. The shift in their positioning showed the effect of bending on the DNA. In total, in the complex with GCN4-bZIP, the ATF/CREB site is bent by (25 ± 2)° and the AP-1 site by (20 ± 2)° toward the minor groove.<ref name="bind"/> AP-1 refers to a group of activator proteins that bind to the same 9 base pair semi-palindromic region for transcription activation. ATF/CREB refers to a fully palendromic region. The AP-1 site sequence is 5′-ATGACTCAT-3′, and the ATF/CREB site is 5′-ATGACGTCAT-3′<ref> Hockings, S. C.; Kahn, J. D.; Crothers, D. M. PNAS February 17, 1998 vol. 95 no. 4 1410-1415 </ref>.		The basic region binding domain of GCN4 inserts itself into the <scene name='Tom_Sandbox/Major_groove/1'>Major Groove</scene> of the DNA. This causes a shift in bothe the AP-1 and ATF/CREB binding sites of DNA<ref name="bind">PMID:15459288</ref>. This was determined by adding fluorophores to the end of U shaped DNA segments with the specific binding sites in the centers. The shift in their positioning showed the effect of bending on the DNA. In total, in the complex with GCN4-bZIP, the ATF/CREB site is bent by (25 ± 2)° and the AP-1 site by (20 ± 2)° toward the minor groove.<ref name="bind"/> AP-1 refers to a group of activator proteins that bind to the same 9 base pair semi-palindromic region for transcription activation. ATF/CREB refers to a fully palendromic region. The AP-1 site sequence is 5′-ATGACTCAT-3′, and the ATF/CREB site is 5′-ATGACGTCAT-3′<ref> Hockings, S. C.; Kahn, J. D.; Crothers, D. M. PNAS February 17, 1998 vol. 95 no. 4 1410-1415 </ref>.