Tom Sandbox: Difference between revisions
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At every d and d' we have a Leucine, while at the a and a' locations we typically see valine. <ref name="Voet" /> In the opposite positions one tends to see more charged and polar residues. The Leucine and Valine repeats create a strongly hydrophobic region between the two alpha helices. This clearly shows the relationship between coils and shown below, we can see that for the two helices to be symmetrical, the Leucines must not in fact be oriented as a zipper, but on the same levels. | At every d and d' we have a Leucine, while at the a and a' locations we typically see valine. <ref name="Voet" /> In the opposite positions one tends to see more charged and polar residues. The Leucine and Valine repeats create a strongly hydrophobic region between the two alpha helices. This clearly shows the relationship between coils and shown below, we can see that for the two helices to be symmetrical, the Leucines must not in fact be oriented as a zipper, but on the same levels. | ||
[[Image:Asymmetric_helices.jpg|center| | [[Image:Asymmetric_helices.jpg|center|400px]] | ||
'''Image 2: The figure above comes from the paper - Secondary Structure of a Leucine Zipper Determined by NMR.<ref name="abc"/>''' | '''Image 2: The figure above comes from the paper - Secondary Structure of a Leucine Zipper Determined by NMR.<ref name="abc"/> The caption was left for clarification.''' | ||
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The basic region binding domain of GCN4 causes a shift in bothe the AP-1 and ATF/CREB binding sites of DNA<ref>PMID:15459288</ref>. | The basic region binding domain of GCN4 causes a shift in bothe the AP-1 and ATF/CREB binding sites of DNA<ref name="bind">PMID:15459288</ref>. This was determined by adding fluorophores to the end of U shaped DNA segments with the specific binding sites in the centers. The shift in their positioning showed the effect of bending on the DNA. In total, in the complex with GCN4-bZIP, the ATF/CREB site is bent by (25 ± 2)° and the AP-1 site by (20 ± 2)° toward the minor groove.<ref name="bind"/> | ||