1odh: Difference between revisions
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[[Image:1odh.gif|left|200px]]<br /><applet load="1odh" size=" | [[Image:1odh.gif|left|200px]]<br /><applet load="1odh" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1odh, resolution 2.85Å" /> | caption="1odh, resolution 2.85Å" /> | ||
'''STRUCTURE OF THE GCM DOMAIN BOUND TO DNA'''<br /> | '''STRUCTURE OF THE GCM DOMAIN BOUND TO DNA'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
1ODH is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=AC1:Zn Binding Site For Chain A'>AC1</scene>. Full crystallographic information is available from [http:// | 1ODH is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=AC1:Zn+Binding+Site+For+Chain+A'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ODH OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: transcription factor]] | [[Category: transcription factor]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 09:56:31 2008'' | ||
Revision as of 10:56, 3 February 2008
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STRUCTURE OF THE GCM DOMAIN BOUND TO DNA
OverviewOverview
Glia cell missing (GCM) transcription factors form a small family of, transcriptional regulators in metazoans. The prototypical Drosophila GCM, protein directs the differentiation of neuron precursor cells into glia, cells, whereas mammalian GCM proteins are involved in placenta and, parathyroid development. GCM proteins share a highly conserved 150 amino, acid residue region responsible for DNA binding, known as the GCM domain., Here we present the crystal structure of the GCM domain from murine GCMa, bound to its octameric DNA target site at 2.85 A resolution. The GCM, domain exhibits a novel fold consisting of two domains tethered together, by one of two structural Zn ions. We observe the novel use of a beta-sheet, in DNA recognition, whereby a five- stranded beta-sheet protrudes into the, major groove perpendicular to the DNA axis. The structure combined with, mutational analysis of the target site and of DNA-contacting residues, provides insight into DNA recognition by this new type of Zn-containing, DNA-binding domain.
About this StructureAbout this Structure
1ODH is a Protein complex structure of sequences from Mus musculus with as ligand. Known structural/functional Site: . Full crystallographic information is available from OCA.
ReferenceReference
Structure of the GCM domain-DNA complex: a DNA-binding domain with a novel fold and mode of target site recognition., Cohen SX, Moulin M, Hashemolhosseini S, Kilian K, Wegner M, Muller CW, EMBO J. 2003 Apr 15;22(8):1835-45. PMID:12682016
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