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Structure of the GCM domain bound to DNAStructure of the GCM domain bound to DNA
Structural highlights
FunctionGCM1_MOUSE Transcription factor that is necessary for placental development (By similarity).[1] Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedGlia cell missing (GCM) transcription factors form a small family of transcriptional regulators in metazoans. The prototypical Drosophila GCM protein directs the differentiation of neuron precursor cells into glia cells, whereas mammalian GCM proteins are involved in placenta and parathyroid development. GCM proteins share a highly conserved 150 amino acid residue region responsible for DNA binding, known as the GCM domain. Here we present the crystal structure of the GCM domain from murine GCMa bound to its octameric DNA target site at 2.85 A resolution. The GCM domain exhibits a novel fold consisting of two domains tethered together by one of two structural Zn ions. We observe the novel use of a beta-sheet in DNA recognition, whereby a five- stranded beta-sheet protrudes into the major groove perpendicular to the DNA axis. The structure combined with mutational analysis of the target site and of DNA-contacting residues provides insight into DNA recognition by this new type of Zn-containing DNA-binding domain. Structure of the GCM domain-DNA complex: a DNA-binding domain with a novel fold and mode of target site recognition.,Cohen SX, Moulin M, Hashemolhosseini S, Kilian K, Wegner M, Muller CW EMBO J. 2003 Apr 15;22(8):1835-45. PMID:12682016[2] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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