Colicin Immunity Protein: Difference between revisions
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[[Image:2k0d.png|left|200px|thumb|Crystal Structure of Colicin immunity protein, [[2d0d]]]] | |||
{{STRUCTURE_2k0d| PDB=2k0d | SIZE=300| SCENE= |right|CAPTION=Colicin-E7 immunity protein, [[2k0d]] }} | |||
Immunity proteins against [[Colicin]]s (EcCIP) are produced by ''E. coli'' alongside the relevant colicin protein (EcCol) to protect the cell from the cytotoxic domain of the colicin. Usually this involves binding to and blocking the active site of the domain, to prevent it from targeting the cells own mechanisms. | Immunity proteins against [[Colicin]]s (EcCIP) are produced by ''E. coli'' alongside the relevant colicin protein (EcCol) to protect the cell from the cytotoxic domain of the colicin. Usually this involves binding to and blocking the active site of the domain, to prevent it from targeting the cells own mechanisms. | ||
Once released from the producing cell, the immunity proteins are no longer needed, as the cytotoxic domain needs to be active once a target cell has been penetrated. Often it is disassociated from the colicin upon binding to an outer membrane receptor on the target cell. | Once released from the producing cell, the immunity proteins are no longer needed, as the cytotoxic domain needs to be active once a target cell has been penetrated. Often it is disassociated from the colicin upon binding to an outer membrane receptor on the target cell. | ||
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==3D structure of Colicin immunity protein== | ==3D structure of Colicin immunity protein== | ||
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[[2xgl]] – EcCIPM periplasmic domain<br /> | [[2xgl]] – EcCIPM periplasmic domain<br /> | ||
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Revision as of 11:42, 17 May 2011
Immunity proteins against Colicins (EcCIP) are produced by E. coli alongside the relevant colicin protein (EcCol) to protect the cell from the cytotoxic domain of the colicin. Usually this involves binding to and blocking the active site of the domain, to prevent it from targeting the cells own mechanisms.
Once released from the producing cell, the immunity proteins are no longer needed, as the cytotoxic domain needs to be active once a target cell has been penetrated. Often it is disassociated from the colicin upon binding to an outer membrane receptor on the target cell.
3D structure of Colicin immunity protein3D structure of Colicin immunity protein
Colicin-D immunity proteinColicin-D immunity protein
1tfk, 1tfo, 1v74 - EcCIPD + EcColD catalytic domain
Colicin-E2 immunity proteinColicin-E2 immunity protein
2no8 – EcCIPE2 – NMR
2wpt – EcCIPE2 + EcColE9 (mutant)
Colicin-E3 immunity proteinColicin-E3 immunity protein
3eip – EcCIPE3
1jch – EcCIPE3 + EcColE3
2b5u – EcCIPE3 + EcColE3 (mutant)
1e44 – EcCIPE3 + EcColE3 nuclease domain
Colicin-E5 immunity proteinColicin-E5 immunity protein
2dfx – EcCIPE5 + EcColE5 C-terminal domain
2fhz – EcCIPE5 + EcColE5 residues 74-180
Colicin-E7 immunity proteinColicin-E7 immunity protein
2k0d, 1ayi, 1unk, 1cei – EcCIPE7
1mz8, 7cei – EcCIPE7 + EcColE7 nuclease domain
2jaz, 2jb0, 2jbg, 1znv – EcCIPE7 + EcColE7 nuclease domain (mutant)
2erh, 1ujz – EcCIPE7 (mutant) + EcColE7 (mutant)
Colicin-E8 immunity proteinColicin-E8 immunity protein
Colicin-E9 immunity proteinColicin-E9 immunity protein
1e0h, 1imp, 1imq – EcCIPE9 - NMR
2k5x, 1emv, 1bxi – EcCIPE9 + EcColE9 DNase domain
2vln, 2vlo, 2vlp, 2vlq - EcCIPE9 + EcColE9 DNase domain (mutant)
2gze, 2gzg, 2gzi, 2gzj, 2gzf, 2gyk, 1fr2 - EcCIPE9 (mutant) + EcColE9 DNase domain
3gjn – EcCIPE9 (mutant) + EcColE7 nuclease domain
3gkl – EcCIPE9 (mutant) + EcColE7 nuclease domain
(mutant)
Colicin-M immunity proteinColicin-M immunity protein
2xgl – EcCIPM periplasmic domain