1agn: Difference between revisions
New page: left|200px<br /> <applet load="1agn" size="450" color="white" frame="true" align="right" spinBox="true" caption="1agn, resolution 3.Å" /> '''X-RAY STRUCTURE OF HU... |
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==About this Structure== | ==About this Structure== | ||
1AGN is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]] with ZN, ACT and NAD as [[http://en.wikipedia.org/wiki/ligands ligands]]. The following page contains interesting information on the relation of 1AGN with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb13_1.html Alcohol Dehydrogenase]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.1 1.1.1.1]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1AGN OCA]]. | 1AGN is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]] with ZN, ACT and NAD as [[http://en.wikipedia.org/wiki/ligands ligands]]. The following page contains interesting information on the relation of 1AGN with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb13_1.html Alcohol Dehydrogenase]]. Active as [[http://en.wikipedia.org/wiki/Alcohol_dehydrogenase Alcohol dehydrogenase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.1 1.1.1.1]]. Structure known Active Sites: ZN1, ZN2, ZN3, ZN4, ZN5, ZN6, ZN7 and ZN8. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1AGN OCA]]. | ||
==Reference== | ==Reference== | ||
X-ray structure of human class IV sigmasigma alcohol dehydrogenase. Structural basis for substrate specificity., Xie P, Parsons SH, Speckhard DC, Bosron WF, Hurley TD, J Biol Chem. 1997 Jul 25;272(30):18558-63. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9228021 9228021] | X-ray structure of human class IV sigmasigma alcohol dehydrogenase. Structural basis for substrate specificity., Xie P, Parsons SH, Speckhard DC, Bosron WF, Hurley TD, J Biol Chem. 1997 Jul 25;272(30):18558-63. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9228021 9228021] | ||
[[Category: Alcohol Dehydrogenase]] | [[Category: Alcohol Dehydrogenase]] | ||
[[Category: Alcohol dehydrogenase]] | |||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: oxidoreductase]] | [[Category: oxidoreductase]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 11:52:49 2007'' | ||
Revision as of 12:48, 30 October 2007
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X-RAY STRUCTURE OF HUMAN SIGMA ALCOHOL DEHYDROGENASE
OverviewOverview
The structural determinants of substrate recognition in the human class, IV, or sigmasigma, alcohol dehydrogenase (ADH) isoenzyme were examined, through x-ray crystallography and site-directed mutagenesis. The crystal, structure of sigmasigma ADH complexed with NAD+ and acetate was solved to, 3-A resolution. The human beta1beta1 and sigmasigma ADH isoenzymes share, 69% sequence identity and exhibit dramatically different kinetic, properties. Differences in the amino acids at positions 57, 116, 141, 309, and 317 create a different topology within the sigmasigma, substrate-binding pocket, relative to the beta1beta1 isoenzyme. The, nicotinamide ring of the NAD(H) molecule, in the sigmasigma structure, appears to be twisted relative to its position in the beta1beta1, isoenzyme. In conjunction ... [(full description)]
About this StructureAbout this Structure
1AGN is a [Single protein] structure of sequence from [Homo sapiens] with ZN, ACT and NAD as [ligands]. The following page contains interesting information on the relation of 1AGN with [Alcohol Dehydrogenase]. Active as [Alcohol dehydrogenase], with EC number [1.1.1.1]. Structure known Active Sites: ZN1, ZN2, ZN3, ZN4, ZN5, ZN6, ZN7 and ZN8. Full crystallographic information is available from [OCA].
ReferenceReference
X-ray structure of human class IV sigmasigma alcohol dehydrogenase. Structural basis for substrate specificity., Xie P, Parsons SH, Speckhard DC, Bosron WF, Hurley TD, J Biol Chem. 1997 Jul 25;272(30):18558-63. PMID:9228021
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